Effect of Fibulin-5 on cell proliferation and invasion in human gastric cancer patients

• Published in: Asian Pacific journal of tropical medicine Vol. 7; no. 10; pp. 787 - 791
• Main Authors: Shi, Xiao-Yu, Wang, Liang, Cao, Chun-Hui, Li, Zhi-Yu, Chen, Jian, Li, Chen
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 01.10.2014; Department of General Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China
• Subjects: cancer; Clinical; Clinical significance; Fibulin-5; Gastric; Gastric cancer; Invasion; Proliferation; significance; Proliferation; Fibulin-5; Gastric cancer; Clinical significance; Invasion
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/s1995-7645(14)60137-1

Objective:To explore the effect of Fibulin-5 expression on cell proliferation and invasion in human gastric cancer patients.Methods:Fibulin-5 expression was detected in 56 samples of surgically resected gastric cancer and paired noncancerous tissues using qRT-PCR and immunoblotting.Fibulin-5 was knocked dowu by Fibulin-5 shRNA in MGC-803 cells,then Brdu cell proliferation and transwell invasion assays were used to determine cell proliferation and invasion.Results:The level of Fibulin-5 mRNA in gastric cancer tissues was significantly higher as compared with that in normal tumor-adjacent tissues(P<0.05).Otherwise,the level of Fibulin-5 protein in cancer and noucancerous tissues was consistent with mRNA expression(P<0.05).Fibulin-5 protein expression in tumor tissues with poorly differentiated.lymph node metastasis and advanced TNM tumor stage was significantly higher(P<0.05.respectively).Fibulin-5 was obviously knocked down by Fibulin-5 shRNA(P<0.05).and Fibulin-5 knockdown significantly inhibited cell proliferation and invasion in MGC-803 cells(P<0.05.respectively).Conclusions:The high-expression of Fibulin-5 is associated with the malignant clinicopathologic parameters in gastric cancer and Fibuliu-5 knockdown inhibits cell proliferation and invasion in MGC-803 cells.suggesting Fibulin-5 may act as a key factor in the progression of gastric cancer.