Association of apolipoprotein M and sphingosine-1-phosphate with brown adipose tissue after cold exposure in humans

• Published in: Scientific reports Vol. 12; no. 1; p. 18753
• Main Authors: Borup, Anna, Donkin, Ida, Boon, Mariëtte R., Frydland, Martin, Martinez-Tellez, Borja, Loft, Annika, Keller, Sune H., Kjaer, Andreas, Kjaergaard, Jesper, Hassager, Christian, Barrès, Romain, Rensen, Patrick C. N., Christoffersen, Christina
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.11.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 692/163; 692/53; Adipose tissue; Adipose tissue (brown); Adipose Tissue, Brown - metabolism; Animals; Apolipoproteins; Apolipoproteins M - metabolism; Body fat; Cooling; Energy metabolism; High density lipoprotein; Humanities and Social Sciences; Humans; Life Sciences; Lipid metabolism; Lysophospholipids - metabolism; Metabolism; multidisciplinary; Permeability; Plasma; Plasma levels; Positron emission tomography; Positron Emission Tomography Computed Tomography; Science; Science (multidisciplinary); Sphingosine - metabolism; Sphingosine 1-phosphate
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-21938-2

The HDL-associated apolipoprotein M (apoM) and its ligand sphingosine-1-phosphate (S1P) may control energy metabolism. ApoM deficiency in mice is associated with increased vascular permeability, brown adipose tissue (BAT) mass and activity, and protection against obesity. In the current study, we explored the connection between plasma apoM/S1P levels and parameters of BAT as measured via 18 F-FDG PET/CT after cold exposure in humans. Fixed (n = 15) vs personalized (n = 20) short-term cooling protocols decreased and increased apoM (− 8.4%, P  = 0.032 vs 15.7%, P  < 0.0005) and S1P (− 41.0%, P  < 0.0005 vs 19.1%, P  < 0.005) plasma levels, respectively. Long-term cooling (n = 44) did not affect plasma apoM or S1P levels. Plasma apoM and S1P did not correlate significantly to BAT volume and activity in the individual studies. However, short-term studies combined, showed that increased changes in plasma apoM correlated with BAT metabolic activity (β: 0.44, 95% CI [0.06–0.81], P  = 0.024) after adjusting for study design but not BAT volume (β: 0.39, 95% CI [− 0.01–0.78], P  = 0.054). In conclusion, plasma apoM and S1P levels are altered in response to cold exposure and may be linked to changes in BAT metabolic activity but not BAT volume in humans. This contrasts partly with observations in animals and highlights the need for further studies to understand the biological role of apoM/S1P complex in human adipose tissue and lipid metabolism.