Optimization of oviposition trap settings to monitor populations of Aedes mosquitoes, vectors of arboviruses in La Reunion

• Published in: Scientific reports Vol. 12; no. 1; pp. 18450 - 14
• Main Authors: Brouazin, Ronan, Claudel, Iris, Lancelot, Renaud, Dupuy, Guillaume, Gouagna, Louis-Clément, Dupraz, Marlène, Baldet, Thierry, Bouyer, Jérémy
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.11.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/158; 631/601/1466; 631/601/18; 692/308/174; Aedes; Aedes aegypti; Aedes albopictus; Animals; Aquatic insects; Arboviruses; Breeding sites; Canopies; Culicidae; Dengue fever; Female; Humanities and Social Sciences; Leaves; Life Sciences; Mosquito Vectors; Mosquitoes; multidisciplinary; Organic matter; Oviposition; Plant Breeding; Plastics - pharmacology; Public health; Reunion; Reunions; Science; Science (multidisciplinary); Trees; Vector-borne diseases; Vectors; Reunion; aedes
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-23137-5

Several dengue epidemics recently occurred in La Reunion, an island harboring two dengue viruses (DVs) vectors: Aedes albopictus , and Ae. aegypti , the former being the main local DV vector. Aedes aegypti shows a peculiar ecology, compared to other tropical populations of the same species. This study aimed to provide researchers and public-health users with locally validated oviposition traps (ovitraps) to monitor Aedes populations. A field experiment was performed in Saint-Joseph to assess the effect of different settings on the detection probability and apparent density of Aedes mosquitoes. Black plastic ovitraps were identified as the best choice. Vacoa trees ( Pandanus utilis ) were the only observed breeding sites for Ae. aegypti , shared with Ae. albopictus . They were the experimental units in a Latin square design with three factors: trap position in the trees (ground vs canopy), oviposition surface in the trap (blotting paper vs. vacoa leaf), and addition of organic matter to the trap water. The latter factor was found unimportant. On the ground, Ae. aegypti eggs were only found with vacoa leaves as the oviposition surface. Their detection and apparent density increased when ovitraps were located in the tree canopy. The main factor for Ae. albopictus was the oviposition surface, with a preference for blotting paper. In all trap settings, their detection was close to 100%. Larval survival was lower for a high egg density, combined with blotting paper as the oviposition surface. When monitoring mixed Aedes populations in La Reunion, we recommend using black plastic ovitraps, placed at 1.50-to-2.00-m high in vacoa trees, with vacoa leaves as the oviposition surface.