Structural insights into p300 regulation and acetylation-dependent genome organisation

• Published in: Nature communications Vol. 13; no. 1; pp. 7759 - 23
• Main Authors: Ibrahim, Ziad, Wang, Tao, Destaing, Olivier, Salvi, Nicola, Hoghoughi, Naghmeh, Chabert, Clovis, Rusu, Alexandra, Gao, Jinjun, Feletto, Leonardo, Reynoird, Nicolas, Schalch, Thomas, Zhao, Yingming, Blackledge, Martin, Khochbin, Saadi, Panne, Daniel
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.12.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 101/28; 101/6; 13/1; 14/19; 14/35; 140/58; 631/337/100/2285; 631/45/173; 631/45/535/878; 82/80; 9/10; Acetylation; Amino acids; Biology; Cancer; Chromatin; Domains; Experiments; Fusion protein; Gene expression; Gene regulation; Genomes; Histones; Humanities and Social Sciences; Life Sciences; Ligands; Lysine; Lysine - metabolism; Medical research; multidisciplinary; Mutation; NMR; Nuclear magnetic resonance; Nuclear Proteins - metabolism; Nuts; Proteins; Science; Science (multidisciplinary); Signal transduction; Transcription activation; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-35375-2

Histone modifications are deposited by chromatin modifying enzymes and read out by proteins that recognize the modified state. BRD4-NUT is an oncogenic fusion protein of the acetyl lysine reader BRD4 that binds to the acetylase p300 and enables formation of long-range intra- and interchromosomal interactions. We here examine how acetylation reading and writing enable formation of such interactions. We show that NUT contains an acidic transcriptional activation domain that binds to the TAZ2 domain of p300. We use NMR to investigate the structure of the complex and found that the TAZ2 domain has an autoinhibitory role for p300. NUT-TAZ2 interaction or mutations found in cancer that interfere with autoinhibition by TAZ2 allosterically activate p300. p300 activation results in a self-organizing, acetylation-dependent feed-forward reaction that enables long-range interactions by bromodomain multivalent acetyl-lysine binding. We discuss the implications for chromatin organisation, gene regulation and dysregulation in disease. Here the authors use structural analyses to show that an intrinsically disordered transcription activation domain in the oncogene BRD4-NUT binds to and activates p300. This in-turn drives formation of higher-order, acetylation-dependent chromatin condensates.