Optical and analytical investigations on dengue virus rapid diagnostic test for IgM antibody detection

• Published in: Medical & biological engineering & computing Vol. 53; no. 8; pp. 679 - 687
• Main Authors: Jahanshahi, Peyman, Sekaran, Shamala Devi, Adikan, Faisal Rafiq Mahamd
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2015; Springer Nature B.V
• Subjects: Analysis; Antibodies; Antibodies, Viral - blood; Antigens; Binding; Bioengineering; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Biosensors; Boundary value problems; Categories; Chromatography; Computer Applications; Computer simulation; Dengue - diagnosis; Dengue - immunology; Dengue fever; Dengue virus; Dengue Virus - immunology; Dengue Virus - isolation & purification; Diagnostic tests; Gold; Hospitals; Human Physiology; Humans; Imaging; Immunoglobulin M - blood; Laboratories; Ligands; Malaysia; Mathematical analysis; Medical; Medical research; Original Article; Patients; Radiology; Reagent Kits, Diagnostic - virology; Sensors; Studies; Surface Plasmon Resonance - methods; Malaysia; Surface plasmon resonance; Analytical analysis; Biosensors; Numerical model; Dengue rapid diagnostic test
• ISSN: 0140-0118; 1741-0444
• DOI: 10.1007/s11517-015-1262-2

Evaluation of binding between analytes and its relevant ligands on surface plasmon resonance (SPR) biosensor is of considerable importance for accurate determination and screening of an interference in immunosensors. Dengue virus serotype 2 was used as a case study in this investigation. This research work compares and interprets the results obtained from analytical analysis with the experimental ones. Both the theoretical calculations and experimental results are verified with one sample from each category of dengue serotypes 2 (low, mid, and high positive), which have been examined in the database of established laboratorial diagnosis. In order to perform this investigation, the SPR angle variations are calculated, analyzed, and then validated via experimental SPR angle variations. Accordingly, the error ratios of 5.35, 6.54, and 3.72 % were obtained for the low-, mid-, and high-positive-specific immune globulins of patient serums, respectively. In addition, the magnetic fields of the biosensor are numerically simulated to show the effect of different binding mediums.