Production and assay of cellulolytic enzyme activity of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya abbatoir, Indonesia

• Published in: Veterinary World Vol. 8; no. 3; pp. 367 - 371
• Main Authors: Lokapirnasari, W P, Nazar, D S, Nurhajati, T, Supranianondo, K, Yulianto, A B
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01.03.2015
• Subjects: 4)-β -D-glucanase; endo-; Enterobacter cloacae WPL 214; exo-; β-glucosidase; endo-(1,4)-β-D-glucanase; exo-(1,4)-β-D-glucanase; Enterobacter cloacae WPL 214; β-glucosidase
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2015.367-371

This study aims to produce and assay cellulolytic enzyme activity (endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase, and β-glucosidase, at optimum temperature and optimum pH) of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya Abbatoir, Indonesia. To produce enzyme from a single colony of E. cloacae WPL 214, 98 × 10(10) CFU/ml of isolates was put into 20 ml of liquid medium and incubated in a shaker incubator for 16 h at 35°C in accordance with growth time and optimum temperature of E. cloacae WPL 214. Further on, culture was centrifuged at 6000 rpm at 4°C for 15 min. Pellet was discarded while supernatant containing cellulose enzyme activity was withdrawn to assay endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase, and β-glucosidase. Cellulase enzyme of E. cloacae WPL 214 isolates had endoglucanase activity of 0.09 U/ml, exoglucanase of 0.13 U/ml, and cellobiase of 0.10 U/ml at optimum temperature 35°C and optimum pH 5. E. cloacae WPL 214 isolated from bovine rumen fluid waste produced cellulose enzyme with activity as cellulolytic enzyme of endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase and β-glucosidase.