Secretion Mode and Subcellular Localization of Human Interferon-β Exogenously Expressed in Porcine Renal Epithelial LLC-PK1 Cells

• Published in: Biological & pharmaceutical bulletin Vol. 27; no. 10; pp. 1653 - 1655
• Main Authors: Nishio, Teruko, Maruyama, Masato, Yoshida, Toyokazu, Watanabe, Yoshihiko, Nishikawa, Makiya, Takakura, Yoshinobu
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 01.10.2004
• Subjects: Animals; Cell Polarity; epithelial cell; Gene Expression; Green Fluorescent Proteins - genetics; Humans; interferon; Interferon-beta - genetics; Interferon-beta - metabolism; LLC-PK1; LLC-PK1 Cells; polarity; Protein Transport; secretory protein; Swine; trans-Golgi Network - physiology; Transfection; μ1B
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.27.1653

We have investigated the secretion polarity of human interferon-β (HuIFN-β) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK1. In these cells, stably expressed HuIFN-β was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-β was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-β revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-β is regulated by a post trans-Golgi network in a cell type-dependent manner.