Multi-omics phenotyping of the gut-liver axis reveals metabolic perturbations from a low-dose pesticide mixture in rats

• Published in: Communications biology Vol. 4; no. 1; pp. 471 - 14
• Main Authors: Mesnage, Robin, Teixeira, Maxime, Mandrioli, Daniele, Falcioni, Laura, Ibragim, Mariam, Ducarmon, Quinten Raymond, Zwittink, Romy Daniëlle, Amiel, Caroline, Panoff, Jean-Michel, Bourne, Emma, Savage, Emanuel, Mein, Charles A., Belpoggi, Fiorella, Antoniou, Michael N.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14.04.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 38/91; 631/45/320; 692/499; Azoxystrobin; Bacteriology; Biochemistry; Biology; Biomedical and Life Sciences; Body weight; Chlorpyrifos; Community composition; CpG islands; DNA methylation; Dosage; Food and Nutrition; Genomes; Glyphosate; Health risk assessment; Health risks; Histopathology; Imidacloprid; Laboratory animals; Life Sciences; Liver; Metabolism; Metabolomics; Metagenomics; Microbiology and Parasitology; Mycology; Nicotinamide; Oxidative stress; Pesticides; Phenotyping; Pollutants; Steroid hormones; Thiabendazole; Toxicity; Tryptophan
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-021-01990-w

Health effects of pesticides are not always accurately detected using the current battery of regulatory toxicity tests. We compared standard histopathology and serum biochemistry measures and multi-omics analyses in a subchronic toxicity test of a mixture of six pesticides frequently detected in foodstuffs (azoxystrobin, boscalid, chlorpyrifos, glyphosate, imidacloprid and thiabendazole) in Sprague-Dawley rats. Analysis of water and feed consumption, body weight, histopathology and serum biochemistry showed little effect. Contrastingly, serum and caecum metabolomics revealed that nicotinamide and tryptophan metabolism were affected, which suggested activation of an oxidative stress response. This was not reflected by gut microbial community composition changes evaluated by shotgun metagenomics. Transcriptomics of the liver showed that 257 genes had their expression changed. Gene functions affected included the regulation of response to steroid hormones and the activation of stress response pathways. Genome-wide DNA methylation analysis of the same liver samples showed that 4,255 CpG sites were differentially methylated. Overall, we demonstrated that in-depth molecular profiling in laboratory animals exposed to low concentrations of pesticides allows the detection of metabolic perturbations that would remain undetected by standard regulatory biochemical measures and which could thus improve the predictability of health risks from exposure to chemical pollutants. Using a multi-omics platform, Mesnage et al present an extensive dataset that reports the effects of low-dose pesticides frequently detected in food on Sprague-Dawley rats. This study suggests potential metabolic biomarkers that may predict health risks from exposure to chemical pollutants.