Viability of ex-vivo myography as a diagnostic tool for rectus abdominis muscle electrical activity collected at Cesarean section within a diamater cohort study

• Published in: Biomedical engineering online Vol. 21; no. 1; pp. 1 - 76
• Main Authors: Reyes, David R. A, Barbosa, Angelica M. P, Juliana, Floriano F, Sofia, Quiroz B. C. V, Costa, Sarah M. B, Hallur, Raghavendra L. S, Enriquez, Eusebio M. A, Oliveira, Rafael G, de Souza Rossignolli, Patricia, Pedroni, Cristiane Rodrigues, Alves, Fernanda C. B, Garcia, Gabriela A, Abbade, Joelcio F, Carvalho, Carolina N. F, Sobrevia, Luis, Rudge, Marilza V. C, Calderon, Iracema I. M. P, Souza, F. P, Lehana, T, Graeff, C. F. O, Magalhães, C. G, Costa, R. A. A, Lima, S. A. M, Rodrigues, M. R. K, Felisbino, S. L, Barbosa, W. F, Campos, F. J, Bossolan, G, Corrente, J. E, Nunes, H. R. C, Rossignoli, P. S, Atallah, Ã. N, Jármy-Di Bella, Z. I, Uchôa, S. M. M, Duarte, M. A. H, Mareco, E. A, Sakalem, M. E, Martinho, N. M, Bussaneli, D. G, Orlandi, M. I. G, Pascon, C, Dangió, T. D, Piculo, F, Prata, G. M, Avramidis, R. E, Magyori, A. B. M, Nava, G. T. A, Caldeirão, T. C. D, Shetty, R. H. L, Marcondes, J. P. C, Takemoto, M. L. S, Prudencio, C. B, Pinheiro, F. A, Sartorao Filho, C. I, Quiroz, S. B. C. V, Pascon, T, Nunes, S. K, Catinelli, B. B, Reis, F. V. D. S, Menezes, M. O, Santos, N. J, Takano, L, Carr, A. M, Iamundo, L. F, Bassin, H. C. M, Barbosa, V. P, Jacomin, M, Silva, A. J. B, Lourenço, I. O, Marosticadesá, J, Caruso, I. P, Rasmussen, L. T, Nogueira, V. K. C, Ribeiro-Paes, J. T, França, D. C. H, Bastos, H. V. M, Heliodoro, M. L. A, Kuroda, M. N, Carvalho, H. L
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 14.10.2022; BioMed Central; BMC
• Subjects: Abdomen; Biopsy; C-section; Cesarean section; Cohort analysis; Complications and side effects; Contractility; Diabetes; Electromyography; Force; Health aspects; Morphology; Muscle contraction; Muscle fatigue; Muscles; Myography; Physiological aspects; Physiological responses; Physiology; Pregnancy; Rectus abdominis muscle; Skeletal muscle; Striated muscle; Tendons; Ultrasonic imaging; Womens health; Brazil
• ISSN: 1475-925X; 1475-925X
• DOI: 10.1186/s12938-022-01042-2

Ex-vivo myography enables the assessment of muscle electrical activity response. This study explored the viability of determining the physiological responses in muscles without tendon, as rectus abdominis muscle (RAM), through ex-vivo myography to assess its potential as a diagnostic tool. All tested RAM samples (five different samples) show patterns of electrical activity. A positive response was observed in 100% of the programmed stimulation. RAM 3 showed greater weight (0.47 g), length (1.66 cm), and width (0.77 cm) compared to RAM 1, RAM 2, RAM 4 and RAM 5 with more sustained electrical activity over time, a higher percentage of fatigue was analyzed at half the time of the electrical activity. The order of electrical activity (Mn) was RAM 3 > RAM 5 > RAM 1 > RAM 4 > RAM 2. No electrical activity was recorded in the Sham group. This study shows that it is feasible to assess the physiological responses of striated muscle without tendon as RAM, obtained at C-section, under ex vivo myography. These results could be recorded, properly analyzed, and demonstrated its potential as a diagnostic tool for rectus abdominis muscle electrical activity.