Cloning and Characterization of K562 Cells on Hemoglobin Synthetic Activity

• Published in: Biological & pharmaceutical bulletin Vol. 16; no. 6; pp. 548 - 551
• Main Authors: SASAKI, Dai, KOSUNAGO, Satoshi, HIRANO, Junko, MATSUDA, Keiichiroh, HATA, Yoko, KOMIYAMA, Yoshimitsu, MIKAMI, Takeshi, MATSUMOTO, Tatsuji, SUZUKI, Masuko
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1993; Maruzen
• Subjects: Animal cells; Antigens, CD - biosynthesis; Antigens, CD - immunology; Antigens, Surface - biosynthesis; Biological and medical sciences; Cell cultures. Hybridization. Fusion; Cell Cycle; Cell Differentiation; cell growth; Clone Cells - metabolism; cloning; Fundamental and applied biological sciences. Psychology; Glycophorin - biosynthesis; glycophorin A; hemoglobin; Hemoglobins - biosynthesis; Heptanoates - pharmacology; Humans; K562 human leukemic cell line; Leukemia, Experimental - metabolism; Micromanipulation; Molecular and cellular biology; Staining and Labeling; Tumor Cells, Cultured - metabolism; Transmembrane protein; Erythroleukemia; Human; Cell proliferation; Cell cloning; Cell culture; Hemoprotein; Established cell line; Hemoglobin; Cell differentiation; Metabolism
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.16.548

Two clones of the K562 human leukemic cell line were isolated according to hemoglobin (Hb) expression. One clone was expressed less than 5% (K562-L) and the other more than 90% (K562-H). The two clones did not exhibit any difference in cell growth or cell cycle. However, the Hb expression of K562-H cells was reduced by succinylacetone (S.A.). The above results suggested that the difference in the Hb production of K562-L and K562-H cells depended on the heme synthetic activity. On the other hand, glycophorin A was expressed to a greater extent on K562-L cells than on K562-H cells. These findings suggested that heme synthesis and the expression of glycophorin A on K562 cells were not always related. The CD11b and the CD61 were also expressed to a greater extent on K562-L cells than on K562-H cells, but the CD34 was not expressed on these cells.