The ID NOW COVID-19, a high-speed high-performance assay

The ID NOW COVID-19 assay is a promising tool for the rapid identification of COVID-19 patients. However, its performances were questioned. We evaluate the ID NOW COVID-19 in comparison to a reference RT-PCR using a collection of 48 fresh nasopharyngeal swabs sampled on universal transport media (UT...

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Published inEuropean journal of clinical microbiology & infectious diseases Vol. 40; no. 9; pp. 2041 - 2045
Main Authors Farfour, Eric, Asso-Bonnet, M., Vasse, M.
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2021
Springer Nature B.V
Springer Verlag
Subjects
Biomedical and Life Sciences
Biomedicine
Brief Report
Coronaviruses
COVID-19
Epidemiology
Gene sequencing
Genetic analysis
Genetic diversity
Genetic screening
Genetic variance
Genomes
High speed
Internal Medicine
Life Sciences
Medical Microbiology
Polymerase chain reaction
Transport media
Whole genome sequencing
COVID-19
SARS-CoV-2
LAMP
NEAR
Isothermal amplification
RT-PCR
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Summary:The ID NOW COVID-19 assay is a promising tool for the rapid identification of COVID-19 patients. However, its performances were questioned. We evaluate the ID NOW COVID-19 in comparison to a reference RT-PCR using a collection of 48 fresh nasopharyngeal swabs sampled on universal transport media (UTM). Only 2 false negatives of the ID NOW COVID-19 were identified. They display PCR cycle threshold values of 37.5 and 39.2. The positive percent agreement and the negative percent agreement were 94.9% and 100%, respectively. The Kappa value was 0.88. The ID NOW COVID-19 combines high-speed and accurate processing. Using UTM, the ID NOW COVID-19 could be repeated in the case of invalid result. Further analyses, such as screening of genetic variants or genome sequencing, could also be performed with the same sample. As for all tests, the results should be interpreted according to clinical and epidemiological context.
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ISSN:0934-9723
1435-4373
1435-4373
DOI:10.1007/s10096-021-04243-0