Label-free optical quantification of structural alterations in Alzheimer’s disease

We present a wide-field quantitative label-free imaging of mouse brain tissue slices with sub-micrometre resolution, employing holographic microscopy and an automated scanning platform. From the measured light field images, scattering coefficients and anisotropies are quantitatively retrieved by usi...

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Published inScientific reports Vol. 6; no. 1; p. 31034
Main Authors Lee, Moosung, Lee, Eeksung, Jung, JaeHwang, Yu, Hyeonseung, Kim, Kyoohyun, Yoon, Jonghee, Lee, Shinhwa, Jeong, Yong, Park, YongKeun
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 03.08.2016
Nature Publishing Group
Subjects
631/1647/328/1650
639/624/1111/55
Alzheimer's disease
Animal tissues
Brain slice preparation
Humanities and Social Sciences
multidisciplinary
Neurodegenerative diseases
Neuroimaging
Scanning
Science
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Summary:We present a wide-field quantitative label-free imaging of mouse brain tissue slices with sub-micrometre resolution, employing holographic microscopy and an automated scanning platform. From the measured light field images, scattering coefficients and anisotropies are quantitatively retrieved by using the modified the scattering-phase theorem, which enables access to structural information about brain tissues. As a proof of principle, we demonstrate that these scattering parameters enable us to quantitatively address structural alteration in the brain tissues of mice with Alzheimer’s disease.
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Present address: Department of Neurology, Seoul National University Bundang Hospital, 82, Gumi-ro 173 Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Republic of Korea.
These authors contributed equally to this work.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep31034