Proteomic analysis of temperature stress in plants

• Published in: Proteomics (Weinheim) Vol. 10; no. 4; pp. 828 - 845
• Main Authors: Neilson, Karlie A, Gammulla, C. Gayani, Mirzaei, Mehdi, Imin, Nijat, Haynes, Paul A
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.02.2010; WILEY-VCH Verlag; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: 2-DE; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Electrophoresis, Gel, Two-Dimensional; Fundamental and applied biological sciences. Psychology; Isotope Labeling; Miscellaneous; Plant Physiological Phenomena; Plant Proteins - analysis; Plant Proteins - chemistry; Plant proteomics; Proteins; Proteomics - methods; Quantitative proteomics; Shotgun proteomics; Staining and Labeling; Temperature; Temperature stress; Temperature; Quantitative proteomics; Temperature stress; Proteomics; Plant proteomics; 2-DE; Shotgun proteomics; Stress; Quantitative analysis
• ISSN: 1615-9853; 1615-9861; 1615-9861
• DOI: 10.1002/pmic.200900538

In this review we examine current approaches used for proteomic analysis of temperature stress in plants. Rapid advances in this field in recent years are discussed, including metabolic, chemical and isotopic labeling, and label-free quantitative techniques. These are compared and contrasted with well-established methods such as 2-DE approaches. Examples of applications of various methods are presented, and technical difficulties and limitations of each are also considered. Results of previous studies are examined in detail, and commonly occurring temperature stress response proteins are collated. We conclude that technical advances, and improvements in genome sequence availability, will have an ever increasing impact on our understanding of molecular mechanisms of stress response in plants.