Multiple analytical methods for identification and quality evaluation of Fritillariae Thunbergii Bulbus based on biological single molecules by high-performance liquid chromatography

• Published in: Journal of separation science Vol. 39; no. 18; pp. 3536 - 3543
• Main Authors: Zhang, Chao, Sun, Lei, Chen, Rong, Su, Jing-hua, Zhang, Hua-feng, Gu, Bing-ren, Xing, Yi-wen, Xue, Man
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.09.2016; Wiley Subscription Services, Inc
• Subjects: Adulterants; Amino acids; Chemometrics; Chromatography; Chromatography, High Pressure Liquid - methods; Cluster analysis; Drugs, Chinese Herbal - chemistry; Fingerprint analysis; Flowers - chemistry; Fritillaria - chemistry; Fritillariae Thunbergii Bulbus; High performance liquid chromatography; Identification methods; Mathematical analysis; Molecular chains; Nucleosides; Principal components analysis; Quality assessment; Quality Control; Quantitative analysis; Similarity; Traditional Chinese medicine; Traditional Chinese medicine; Fritillariae Thunbergii Bulbus; Fingerprint analysis; High-performance liquid chromatography; Chemometrics
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201600356

Quantitative analysis for biological single molecules in Fritillariae Thunbergii Bulbus and identification method by chemometrics was established by using high‐performance liquid chromatography. Amino acids, nucleosides, and nucleobases were quantitatively analyzed, and 11 peaks were selected for species identification. A Common pattern was established for chemometrics, and then similarity analysis, principal component analysis, and hierarchical cluster analysis heatmap were applied, and the results indicated that species were ideally identified from the adulterants as Fritillariae Cirrhosae Bulbus. This evaluation method was valuable for further quality control to select the characteristic components.