散发性乳腺癌中DNMT3a、DNMT3b表达与ERα基因启动子甲基化状态及蛋白表达的关系

目的探讨散发性乳腺癌中DNA甲基转移酶（DNMT）3a、DNMT3b蛋白表达情况及其与雌激素受体（ER）α基因启动子甲基化状态及蛋白表达的关系。方法选取180例散发性乳腺癌与30例乳腺纤维腺瘤组织,采用免疫组化法检测组织中DNMT3a、DNMT3b蛋白的表达情况,甲基化特异性PCR检测97例散发性乳腺癌中ERα基因启动子的甲基化状态。结果乳腺纤维腺瘤与乳腺癌组织中的DNMT3a、DNMT3b蛋白阳性表达率差异无统计学意义;Ⅲ-Ⅳ期乳腺癌组织的DNMT3a、DNMT3b水平高于Ⅰ-Ⅱ期,有淋巴结转移者的DNMT3a表达水平高于无淋巴结转移者（均P〈0.05）;97例乳腺癌中有39例（40.2%）...

Full description

Saved in:
Bibliographic Details
Published in天津医药 Vol. 43; no. 5; pp. 500 - 504
Main Author 王勇 王晓东 陈文捷 于兆进 吴慧哲 赵琳 魏敏杰
Format Journal Article
LanguageChinese
Published 中国医科大学药学院办公室 110013%中国医科大学药学院药理学教研室 2015
Subjects
DNA甲基化
DNMT3a
DNMT3b
ERα
启动区（遗传学）
散发性乳腺癌
DNA甲基化
promoter regions (genetics)
ERα
DNMT3a
DNMT3b
启动区(遗传学)
DNA methylation
sporadic breast cancer
散发性乳腺癌
Online AccessGet full text
ISSN0253-9896
DOI10.11958/j.issn.0253-9896.2015.05.014

Cover

More Information
Summary:目的探讨散发性乳腺癌中DNA甲基转移酶（DNMT）3a、DNMT3b蛋白表达情况及其与雌激素受体（ER）α基因启动子甲基化状态及蛋白表达的关系。方法选取180例散发性乳腺癌与30例乳腺纤维腺瘤组织,采用免疫组化法检测组织中DNMT3a、DNMT3b蛋白的表达情况,甲基化特异性PCR检测97例散发性乳腺癌中ERα基因启动子的甲基化状态。结果乳腺纤维腺瘤与乳腺癌组织中的DNMT3a、DNMT3b蛋白阳性表达率差异无统计学意义;Ⅲ-Ⅳ期乳腺癌组织的DNMT3a、DNMT3b水平高于Ⅰ-Ⅱ期,有淋巴结转移者的DNMT3a表达水平高于无淋巴结转移者（均P〈0.05）;97例乳腺癌中有39例（40.2%）发生ERα基因启动子甲基化,DNMT3a蛋白阳性表达与ERα基因甲基化呈正相关（rs=0.250）;DNMT3a蛋白表达对患者的总体生存期（OS）有显著影响（P=0.035）,对无病生存期（DFS）无明显影响（P=0.064）,DNMT3b蛋白表达对患者的OS和DFS无影响（P分别为0.914、0.961）。结论DNMT3a、DNMT3b阳性表达与乳腺癌侵袭转移、病程进展、预后相关;DNMT3a与ERα基因启动子甲基化状态呈正相关;抑制DNMT3a、DNMT3b蛋白可能有利于散发性乳腺癌的预防和治疗。
Bibliography:Objective To investigate the correlationship between DNMT3 a,DNMT3 b protein expressions and the stateof promoter methylation of ERα gene and ERα protein expression in the development of sporadic breast cancer.Methods A total of 180 patients with sporadic breast cancer and 30 patients with breast fibroadenoma were included in this study.Theexpressions of DNMT3 a and DNMT3 b protein were detected by immunohistochemical method.The state of promoter methyla-tion of ERα gene was detected by methylation specific PCR in 97 patients with sporadic breast cancer.Results There wereno significant differences in positive expression rates of DNMT3 a and DNMT3 b protein between breast fibroadenoma andbreast cancer.There were higher expression levels of DNMT3 a and DNMT3 b in breast cancer patient of Ⅲ~Ⅳ stages thanthose ofⅠ~Ⅱstages.The expression of DNMT3 a was significantly higher in patients with lymph node metastasis than that ofpatients without lymph node metastasis（P〈0.05）.Of 97 cases of breast cancer patients,ERα gen
ISSN:0253-9896
DOI:10.11958/j.issn.0253-9896.2015.05.014