Influence of resin modified glass ionomer cement incorporating protein-repellent and antimicrobial agents on supragingival microbiome around brackets: an in-vivo split-mouth 3-month study

• Published in: PeerJ (San Francisco, CA) Vol. 11; p. e14820
• Main Authors: Ma, Yansong, Su, Chengjun, Yang, Hao, Xu, Hockin H K, Bai, Yuxing, Xu, Yan, Che, Xiaoxia, Zhang, Ning
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 06.02.2023; PeerJ, Inc; PeerJ Inc
• Subjects: Adhesives; Aluminum Silicates; Ammonium; Analysis; Anti-Infective Agents; Antibiotics; Antimicrobial activity; Antimicrobial agents; Bacteria; Biofilms; Cement; Composite materials; Decalcification; Dental cement; Dental enamel; Dentistry; Enamel; Enamel demineralization; Fluorides; Gingival index; Glass Ionomer Cements - therapeutic use; Humans; Microbiology; Microbiomes; Mouth; Next-generation sequencing; Oral hygiene; Oral microbiome; Orthodontic adhesive; Orthodontics; Patient compliance; Patient satisfaction; Phosphorylcholine; Plaque index; Prevention; Prevotella; Protein repellent; Proteins; Resins, Plant; RNA; RNA, Ribosomal, 16S; rRNA 16S; Streptococcus; Streptococcus mutans; Teeth; Japan; Beijing China; China; Protein repellent; Antimicrobial activity; Enamel demineralization; Orthodontic adhesive; Oral microbiome
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.14820

To explore the influence of resin modified glass ionomer cement (RMGIC) adhesives containing protein-repellent and quaternary ammonium salt agents on supragingival microbiome, enamel and gingival health around brackets. Ten patients (21.4 ± 3.5 years) about to receive fixed orthodontics were enrolled in this study. Unilateral upper teeth bonded with RMGIC incorporating 2-Methacryloyloxyethyl phosphorylcholine (MPC) and Dimethylaminohexadecyl methacrylate (DMAHDM) were regarded as experimental group (RMD), while contralateral upper teeth bonded with RMGIC were control group (RMGIC), using a split-mouth design. Supragingival plaque was collected from both groups before treatment (T0), and at 1 month (T1) and 3 months (T2) of treatment. High-throughput sequencing was performed targeting v3-v4 of 16S rRNA gene. and quantification was done by qPCR analysis. Bracket failures, enamel decalcification index (EDI), DIAGNODent scores (Dd), plaque index (PI) and gingival index (GI) were monitored at indicated time points. Within 3 months, alpha and beta diversity of supragingival plaque had no difference between RMGIC and RMD groups. From T0 to T2, the relative abundance of depleted in RMD but remained steady in RMGIC group. , , and became depleted in RMD, and became depleted in RMGIC group but enriched. Quantification of and showed significant difference between RMGIC and RMD groups at T2. Teeth bonded with RMD had significant lower plaque index (PI) and DIAGNODent (Dd) score at T2, compared with teeth bonded with RMGIC ( < 0.05). No difference in bracket failure rate was examined between both groups ( > 0.05). By incorporating MPC and DMAHDM into RMGIC, the material could affect the supragingival microbial composition, inhibit the progress of plaque accumulation as well as the key pathogens and in the early stage of orthodontic treatment.