Bacteriocin typing of Burkholderia (Pseudomonas) solanacearum race 1 of the French West Indies and correlation with genomic variation of the pathogen

• Published in: Applied and Environmental Microbiology Vol. 62; no. 2; pp. 473 - 479
• Main Authors: Frey, P. (INRA, Champenoux, France.), Smith, J.J, Albar, L, Prior, P, Saddler, G.S, Trigalet-Demery, D, Trigalet, A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.02.1996
• Subjects: ADN; AGENT PATHOGENE; AMPLIFICATION CHAINE POLYMERASE; Bacteria; BACTERIOCINAS; BACTERIOCINE; Bacteriology; BACTERIOSE; BACTERIOSIS; Biological and medical sciences; Cellular biology; DNA fingerprints; Ecology, environment; ELECTROFORESIS; ELECTROPHORESE; EMPREINTE ADN; ENFERMEDADES DE LAS PLANTAS; Flowers & plants; Fundamental and applied biological sciences. Psychology; GUADALUPE; GUADELOUPE; HUELLAS GENETICAS ADN; IDENTIFICACION; IDENTIFICATION; Life Sciences; LYCOPERSICON ESCULENTUM; MALADIE DES PLANTES; Microbiology; MUTANT; MUTANTES; NUCLEASAS; NUCLEASE; ORGANISMOS PATOGENOS; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; PSEUDOMONACEAE; PSEUDOMONAS SOLANACEARUM; RACE PHYSIOLOGIQUE; RAZAS FISIOLOGICAS; REACCION DE CADENAS DE POLIMERASA; West Indies; Resistance; Restriction fragment length polymorphism; Typing; Genetic variability; Gene; Genetic marker; Bacteriocin; Bacteria; Isolate; Genetic distance; Burkholderia solanacearum
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.62.2.473-479.1996

Burkholderia solanacearum race 1 isolates indigenous to the French West Indies were characterized by bacteriocin typing and two genomic fingerprinting methods: pulsed-field gel electrophoresis of genomic DNA digested by rare-cutting restriction endonucleases (RC-PFGE) and PCR with primers corresponding to repetitive extragenic palindromic (REP) enterobacterial repetitive intergenic consensus (ERIC) and BOX elements (collectively-known as rep-PCR). The survey comprised 24 reference strains and 65 isolates obtained from a field trial in (Guadeloupe in 1993. Comparison of the data identified RC-PFGE as the most discriminatory method, delineating 17 pulsed-field gel profile types. rep-PCR and bacteriocin typing identified nine rep-PCR profile types and nine bacteriocin groups. Independent determination of similarity coefficients and clustering of RC-PFGE and rep-PCR data identified six groups common to both sets of data that correlated to biovar and bacteriocin groups. Further study of bacteriocin production in planta gave results consistent with in vitro bacteriocin typing. It was observed that spontaneous bacteriocin-resistant mutants exhibited a cross-resistance to other bacteriocins as identified by the typing scheme and that such mutants possessed a selective advantage for growth over isogenic nonmutants in the presence of a bacteriocin. The results are significant in the search for biological control of disease by nonpathogenic mutants of the wild-type organism