In vitro screening of radioprotective properties in the novel glucosylated flavonoids

• Published in: International journal of molecular medicine Vol. 38; no. 5; pp. 1525 - 1530
• Main Authors: Yu, Hao, Haskins, Jeremy S, Su, Cathy, Allum, Allison, Haskins, Alexis H, Salinas, Victoria A, Sunada, Shigeaki, Inoue, Taisuke, Aizawa, Yasushi, Uesaka, Mitsuru, Kato, Takamitsu A
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.11.2016; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Antioxidants; Antioxidants - analysis; Bioflavonoids; Biphenyl Compounds - chemistry; Chemicals; Complications and side effects; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded - drug effects; DNA double-strand breaks; Drug Evaluation, Preclinical; Electrophoresis, Agar Gel; Flavones; Flavonoids; Flavonoids - analysis; Flavonoids - chemistry; Flavonoids - pharmacology; Free Radical Scavengers - chemistry; Free radicals; Genetic testing; glucosylated flavonoids; Glycosylation - drug effects; Health aspects; Medical research; Medicine, Experimental; Medicine, Preventive; Nephelometry and Turbidimetry; Picrates - chemistry; Preventive health services; Radiation-Protective Agents - analysis; Radiation-Protective Agents - chemistry; Radiation-Protective Agents - pharmacology; Radiotherapy; United States > US; Japan
• ISSN: 1107-3756; 1791-244X
• DOI: 10.3892/ijmm.2016.2764

Novel glucosyl flavonoids are developed by the addition of glucose to naturally occurring flavonoids. Flavonoids are known antioxidants that possess radioprotective properties. In order to investigate the radioprotective properties of novel glucosyl flavonoids, in vitro DNA double-strand breaks (DSBs) analysis was carried out. In the present study, Quercetin, Naringenin, and Hesperetin groups of flavonoids included in the natural and novel glucosyl 13 flavonoids were investigated. Flavonoids were mixed with Lambda DNA, and subsequently exposed to gamma-rays. Furthermore, DNA DSB yields were visualized by gel electrophoresis. Quercetin derivatives displayed reduced DNA DSB formation at 10 μM. At a high concentration, the majority of flavonoids displayed radioprotective properties as a reduction of DSB yields. Suppression of DSB formation was confirmed via the molecular combing assay for Quercetin, and three monoglucosyl flavonoids. Glucosylation showed positive effects for radioprotection and monoglucosyl-Rutin showed superior radioprotective properties when compared to monoglucosyl-Naringin and Hesperidin. In addition, Quercetin derivatives had greater total antioxidant capacities and DPPH radical scavenging ability than other flavonoid groups. Since Quercetin, Isoquercetin, and Rutin display poor water solubility, monoglucosyl-Rutin, maltooligosyl-Isoquercetin, and maltooligosyl-Rutin may be better radioprotective agents and easily bioavailable with increased water solubility.