SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23

• Published in: BMC cancer Vol. 24; no. 1; pp. 210 - 10
• Main Authors: Wu, Yongkang, Chen, Weijie, Miao, Huilai, Xu, Tuo
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 15.02.2024; BioMed Central; BMC
• Subjects: Analysis; Anaplastic thyroid cancer; Antibodies; Biotechnology; Cancer therapies; Care and treatment; Cell migration; Cell proliferation; Cell viability; Cells; Diagnosis; Gene expression; Genetic aspects; Health aspects; Immunoprecipitation; KIF23; Kinesin; Medical prognosis; Migration; Penicillin; Proliferation; Proteins; Reverse transcription; RNA polymerase; Scientific equipment and supplies industry; SIRT7; Succinylation; T cells; Thyroid cancer; Thyroid gland; Wound healing; Wounds and injuries; China; United States; United States > US; Japan; Anaplastic thyroid cancer; KIF23; Proliferation; SIRT7; Succinylation; Migration
• ISSN: 1471-2407; 1471-2407
• DOI: 10.1186/s12885-024-11965-9

This study was designed to investigate the regulatory effects of kinesin family member (KIF) 23 on anaplastic thyroid cancer (ATC) cell viability and migration and the underlying mechanism. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to analyze the levels of KIF23 in ATC cells. Besides, the effects of KIF23 and sirtuin (SIRT) 7 on the viability and migration of ATC cells were detected using cell counting kit-8, transwell and wound healing assays. The interaction between SIRT7 and KIF23 was evaluated by co-immunoprecipitation (Co-IP) assay. The succinylation (succ) of KIF23 was analyzed by western blot. The KIF23 expression was upregulated in ATC cells. Silencing of KIF23 suppressed the viability and migration of 8505C and BCPAP cells. The KIF23-succ level was decreased in ATC cells. SIRT7 interacted with KIF23 to inhibit the succinylation of KIF23 at K537 site in human embryonic kidney (HEK)-293T cells. Overexpression of SIRT7 enhanced the protein stability of KIF23 in HEK-293T cells. Besides, overexpression of KIF23 promoted the viability and migration of 8505C and BCPAP cells, which was partly blocked by silenced SIRT7. SIRT7 promoted the proliferation and migration of ATC cells by regulating the desuccinylation of KIF23.