Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation

• Published in: Pharmaceutics Vol. 15; no. 3; p. 828
• Main Authors: Garrigós, Murilo M, Oliveira, Fernando A, Nucci, Mariana P, Mamani, Javier B, Dias, Olívia F M, Rego, Gabriel N A, Junqueira, Mara S, Costa, Cícero J S, Silva, Lucas R R, Alves, Arielly H, Valle, Nicole M E, Marti, Luciana, Gamarra, Lionel F
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.03.2023; MDPI
• Subjects: Bioluminescence; bioluminescence imaging; Bone marrow; bone marrow transplantation; Chemical properties; Chemokines; Cytokines; Einstein, Albert (1879-1955); engraftment; Evaluation; Flow cytometry; Hematology; Hematopoietic stem cells; Humidity; ICP-MS; Laboratory animals; Magnetic properties; Magnetic resonance imaging; Mass spectrometry; Nanoparticles; Stem cells; superparamagnetic iron oxide nanoparticles; Transplantation; Transplants & implants; Brazil; New York; St Louis Missouri; United States > US; engraftment; superparamagnetic iron oxide nanoparticles; bioluminescence imaging; ICP-MS; homing; hematopoietic stem cells; bone marrow transplantation
• ISSN: 1999-4923; 1999-4923
• DOI: 10.3390/pharmaceutics15030828

Bone marrow transplantation is a treatment for a variety of hematological and non-hematological diseases. For the transplant success, it is mandatory to have a thriving engraftment of transplanted cells, which directly depends on their homing. The present study proposes an alternative method to evaluate the homing and engraftment of hematopoietic stem cells using bioluminescence imaging and inductively coupled plasma mass spectrometry (ICP-MS) associated with superparamagnetic iron oxide nanoparticles. We have identified an enriched population of hematopoietic stem cells in the bone marrow following the administration of Fluorouracil (5-FU). Lately, the cell labeling with nanoparticles displayed the greatest internalization status when treated with 30 µg Fe/mL. The quantification by ICP-MS evaluate the stem cells homing by identifying 3.95 ± 0.37 µg Fe/mL in the control and 6.61 ± 0.84 µg Fe/mL in the bone marrow of transplanted animals. In addition, 2.14 ± 0.66 mg Fe/g in the spleen of the control group and 2.17 ± 0.59 mg Fe/g in the spleen of the experimental group was also measured. Moreover, the bioluminescence imaging provided the follow up on the hematopoietic stem cells behavior by monitoring their distribution by the bioluminescence signal. Lastly, the blood count enabled the monitoring of animal hematopoietic reconstitution and ensured the transplantation effectiveness.