Cystic fibrosis mouse model-dependent intestinal structure and gut microbiome
Mice with a null mutation in the cystic fibrosis transmembrane conductance regulator ( Cftr ) gene show intestinal structure alterations and bacterial overgrowth. To determine whether these changes are model-dependent and whether the intestinal microbiome is altered in cystic fibrosis (CF) mouse mod...
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Published in | Mammalian genome Vol. 26; no. 5-6; pp. 222 - 234 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.06.2015
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Mice with a null mutation in the cystic fibrosis transmembrane conductance regulator (
Cftr
) gene show intestinal structure alterations and bacterial overgrowth. To determine whether these changes are model-dependent and whether the intestinal microbiome is altered in cystic fibrosis (CF) mouse models, we characterized the ileal tissue and intestinal microbiome of mice with the clinically common ΔF508
Cftr
mutation (FVB/N
Cftr
tm1Eur
) and with
Cftr
null mutations (BALB/c
Cftr
tm1UNC
and C57BL/6
Cftr
tm1UNC
). Intestinal disease in 12-week-old CF mice, relative to wild-type strain controls, was measured histologically. The microbiome was characterized by pyrosequencing of the V4–V6 region of the 16S rRNA gene and intestinal load was measured by RT-PCR of the 16S rRNA gene. The CF-associated increases in ileal crypt to villus axis distention, goblet cell hyperplasia, and muscularis externa thickness were more severe in the BALB/c and C57BL/6
Cftr
tm1UNC
mice than in the FVB/N
Cftr
tm1Eur
mice. Intestinal bacterial load was significantly increased in all CF models, compared to levels in controls, and positively correlated with circular muscle thickness in CF, but not wild-type, mice. Microbiome profiling identified
Bifidobacterium
and groups of
Lactobacillus
to be of altered abundance in the CF mice but overall bacterial frequencies were not common to the three CF strains and were not correlative of major histological changes. In conclusion, intestinal structure alterations, bacterial overgrowth, and dysbiosis were each more severe in BALB/c and C57BL/6
Cftr
tm1UNC
mice than in the FVB/N
Cftr
tm1Eur
mice. The intestinal microbiome differed among the three CF mouse models. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0938-8990 1432-1777 |
DOI: | 10.1007/s00335-015-9560-4 |