Identifying the Potential Differentially Expressed miRNAs and mRNAs in Osteonecrosis of the Femoral Head Based on Integrated Analysis

• Published in: Clinical interventions in aging Vol. 16; pp. 187 - 202
• Main Authors: Hao, Yangquan, Lu, Chao, Zhang, Baogang, Xu, Zhaochen, Guo, Hao, Zhang, Gaokui
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2021; Taylor & Francis Ltd; Dove; Dove Medical Press
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; Bones; Cell adhesion & migration; Chemokines - metabolism; Cytokines; Datasets; Femur Head - metabolism; Gene expression; Growth factors; Health aspects; Humans; Identification and classification; Messenger RNA; MicroRNA; MicroRNAs - metabolism; mirnas; mrnas; Necrosis; Nuclear Proteins - metabolism; Original Research; Osteonecrosis; osteonecrosis of the femoral head; Physiological aspects; Reelin Protein; RNA, Messenger - metabolism; Signal Transduction - physiology; signaling pathway; China; signaling pathway; miRNAs; mRNAs; osteonecrosis of the femoral head
• ISSN: 1178-1998; 1176-9092; 1178-1998
• DOI: 10.2147/CIA.S289479

Osteonecrosis of the femoral head is a common disease of the hip that leads to severe pain or joint disability. We aimed to identify potential differentially expressed miRNAs and mRNAs in osteonecrosis of the femoral head. The data of miRNA and mRNA were firstly downloaded from the database. Secondly, the regulatory network of miRNAs-mRNAs was constructed, followed by function annotation of mRNAs. Thirdly, an in vitro experiment was applied to validate the expression of miRNAs and targeted mRNAs. Finally, GSE123568 dataset was used for electronic validation and diagnostic analysis of targeted mRNAs. Several regulatory interaction pairs between miRNA and mRNAs were identified, such as hsa-miR-378c-WNT3A/DACT1/CSF1, hsa-let-7a-5p-RCAN2/IL9R, hsa-miR-28-5p-RELA, hsa-miR-3200-5p-RELN, and hsa-miR-532-5p-CLDN18/CLDN10. Interestingly, CLDN10, CLDN18, CSF1, DACT1, IL9R, RCAN2, RELN, and WNT3A had the diagnostic value for osteonecrosis of the femoral head. Wnt signaling pathway (involved WNT3A), chemokine signaling pathway (involved RELA), focal adhesion and ECM-receptor interaction (involved RELN), cell adhesion molecules (CAMs) (involved CLDN18 and CLDN10), cytokine-cytokine receptor interaction, and hematopoietic cell lineage (involved CSF1 and IL9R) were identified. The identified differentially expressed miRNAs and mRNAs may be involved in the pathology of osteonecrosis of the femoral head.