Prevalence, risk and genetic characteristics of drug-resistant tuberculosis in a tertiary care tuberculosis hospital in China

• Published in: Infection and drug resistance Vol. 12; pp. 2457 - 2465
• Main Authors: Zhao, Li-li, Huang, Ming-xiang, Xiao, Tong-yang, Liu, Hai-can, Li, Ma-chao, Zhao, Xiu-qin, Liu, Zhi-guang, Jiang, Yi, Wan, Kang-lin
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.08.2019; Taylor & Francis Ltd; Dove; Dove Medical Press
• Subjects: Antibacterial agents; Antitubercular agents; Capreomycin; Deoxyribonucleic acid; Disease control; Disease prevention; DNA; DNA sequencing; Drug resistance; Drug therapy; drug-resistant tuberculosis; Drugs; Ethambutol; genotype; Genotypes; Genotyping; Hospitals; Infectious diseases; Isoniazid; Laboratories; Medical research; Microbial drug resistance; Multidrug resistance; Mutation; Nucleotides; Ofloxacin; Original Research; Patients; Public health; Rifampin; risk; RpoB protein; Rural areas; Statistical analysis; Streptomycin; Studies; Tuberculosis; China; Beijing China; mutation; risk; drug-resistant tuberculosis; genotype
• ISSN: 1178-6973; 1178-6973
• DOI: 10.2147/IDR.S209971

To explore the prevalence, risk and genetic characteristics of drug-resistant tuberculosis (TB) from a tertiary care TB hospital in China. We carried out a retrospective study including isolates from 189 patients with pulmonary TB at Fuzhou Pulmonary Hospital. All isolates from these patients were subjected to drug susceptibility testing and genotyping. For drug-resistant isolates, DNA sequencing was used to investigate mutations in 12 loci, including , , , , , (nucleotides 388-1084 of rrs), , , , (nucleotides 1158-1674 of rrs), and . Among 189 isolates, 28.6% were resistant to at least one of the seven anti-TB drugs, including isoniazid (INH), rifampin (RIF), streptomycin (STR), ethambutol (EMB), capreomycin (CAP), kanzmycin (KAN) and ofloxacin (OFX). The proportion of multidrug-resistant TB and extensively drug-resistant TB isolates was 9.5% and 1.1%, respectively. Patients in rural areas as well as previously treated patients showed a significantly increased risk of developing drug resistance. In addition, among these isolates, 111 (58.7%) were Beijing genotype strains, 84 (75.7%) of which belonged to modern Beijing sublineage. There was no association between genotype and drug resistance. The most common mutations were 315, 531 43, 306, 1401 and 94. These findings provided additional information of drug-resistant TB in China. Previously treated patients and patients in rural areas should receive greater attention owing to their higher risk of developing drug resistance.