Exosomes derived from cancer-associated fibroblasts promote tumorigenesis, metastasis and chemoresistance of colorectal cancer by upregulating circ_0067557 to target Lin28

• Published in: BMC cancer Vol. 24; no. 1; pp. 64 - 15
• Main Authors: Yang, Cheng, Zhang, Yan, Yan, Mingze, Wang, Jiahao, Wang, Jiaming, Wang, Muhong, Xuan, Yuhong, Cheng, Haiyue, Ma, Jiaao, Chai, Cuicui, Li, Mingzhe, Yu, Zhiwei
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 12.01.2024; BioMed Central; BMC
• Subjects: Analysis; Animals; Antibodies; Apoptosis; Cancer; Cancer-associated fibroblasts; Cancer-Associated Fibroblasts - metabolism; Carcinogenesis - metabolism; Care and treatment; Cell culture; Cell Line, Tumor; Cell proliferation; Cell Proliferation - genetics; Cell Transformation, Neoplastic - metabolism; Chemoresistance; Chemotherapy; Cholecystokinin; circ_0067557; Circular RNA; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - pathology; Complications and side effects; Development and progression; Diagnosis; Drug resistance; Drug Resistance, Neoplasm - genetics; Exosomes; Exosomes - genetics; Exosomes - metabolism; Fibroblasts; Flow cytometry; Genetic aspects; Humans; Hybridization; Lin28A; Lin28B; Metastases; Metastasis; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; Pharmaceutical industry; Phenotypes; Plasmids; Prevention; RNA; RNA, Circular - genetics; RNA, Circular - metabolism; Tumor microenvironment; Tumor Microenvironment - genetics; Tumorigenesis; China; United Kingdom; United States; Japan; Germany; United Kingdom > UK; United States > US; Lin28A; Cancer-associated fibroblasts; Lin28B; Exosomes; Colorectal cancer; circ_0067557
• ISSN: 1471-2407; 1471-2407
• DOI: 10.1186/s12885-023-11791-5

Cancer associated fibroblasts (CAFs) can remodel tumor microenvironment by secreting exosomes. This study aimed to investigate the role of exosomes derived from cancer-associated fibroblasts in colorectal cancer (CRC) progression. Circular RNA (circRNA) array was used to identify differentially expressed circRNAs in exosomes from normal fibroblasts (NFs) and CAFs, and confirmed one differentially expressed circRNA circ_0067557 by real-time PCR. The effect of circ_0067557 on proliferation, metastasis, chemoresistance and apoptosis was verified by wound heal, tranwell, CCK8, sphere-forming and flow cytometry assay. Circ_0067557 expression in exosomes from CAFs was higher than those from NFs. CAF-derived exosomes promoted the proliferation, migration, invasion and chemoresistance of CRC cells while suppressed apoptosis. Silencing of circ_0067557 inhibited malignant phenotypes of CRC cells by targeting Lin28A and Lin28B. Moreover, CAF-derived exosomes enhanced the growth of CRC xenograft tumors. Circ_0067557/Lin28A and Lin28B signal axis may be a potential therapy target for CRC.