Cell-Mediated Hemolytic Activity of Nosocomial Pseudomonas Aeruginosa Strains

• Published in: Bulletin of experimental biology and medicine Vol. 159; no. 2; pp. 258 - 261
• Main Authors: Kuznetsova, M. V., Karpunina, T. I.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2015; Springer; Springer Nature B.V
• Subjects: ADP Ribose Transferases - metabolism; ADP Ribose Transferases - toxicity; Bacterial Proteins - metabolism; Bacterial Proteins - toxicity; Bacterial Toxins - metabolism; Bacterial Toxins - toxicity; Biomedical and Life Sciences; Biomedicine; Cell Adhesion - physiology; Cell Biology; Centrifugation; Cross Infection - physiopathology; DNA Primers - genetics; Erythrocytes - metabolism; Hemoglobins; Hemoglobins - metabolism; Hemolysis - physiology; Humans; Internal Medicine; Laboratory Medicine; Pathology; Polymerase Chain Reaction; Pseudomonas aeruginosa; Pseudomonas aeruginosa - physiology; Pseudomonas Infections - physiopathology; Species Specificity; ExoU; adhesion; cell-mediated hemolysis; ExoS; P. aeruginosa
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-015-2936-3

Cell-mediated hemolysis and adhesion index of nosocomial P. aeruginosa strains were experimentally studied. The highest hemoglobin release was recorded after centrifugation of erythrocyte and bacterial cell suspension preincubated at 37 C. All cultures were referred to highly adherent variants. The relationship between P. aeruginosa adhesion activity and erythrocyte lysis was found only in “passive” cell–cell contact. No correlation between cellassociated hemolysis and hemolysis caused by secreted factors was detected. It seems that the cytotoxicity of the studied P. aeruginosa strains was determined by ExoU and ExoS third type secretion effectors.