Ginsenoside Rb1, salvianolic acid B and their combination modulate gut microbiota and improve glucolipid metabolism in high-fat diet induced obese mice

• Published in: PeerJ (San Francisco, CA) Vol. 9; p. e10598
• Main Authors: Bai, Ying, Bao, Xueli, Mu, Qianqian, Fang, Xin, Zhu, Ruyuan, Liu, Chenyue, Mo, Fangfang, Zhang, Dongwei, Jiang, Guangjian, Li, Ping, Gao, Sihua, Zhao, Dandan
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 03.02.2021; PeerJ, Inc; PeerJ Inc
• Subjects: 16S rDNA sequencing; Blood; Blood sugar; Body weight; Cardiovascular disease; Cholesterol; Deoxyribonucleic acid; Diabetes; Diabetes and Endocrinology; Diet; DNA; Experiments; Fasting; Fat metabolism; Fatty acids; Feces; Ginsenoside Rb1; Ginsenosides; Glucolipid metabolism; Glucose; Glucose tolerance; Glucose tolerance tests; Glycosylated hemoglobin; Gut microbiota; Hemoglobin; High fat diet; Intestinal microflora; Laboratory animals; Laboratory testing; Low density lipoprotein; Medicine; Metabolic disorders; Metabolic Sciences; Metabolites; Microbiology; Microbiota; Microbiota (Symbiotic organisms); Molecular Biology; Obesity; rRNA 16S; Salvianolic acid B; Triglycerides; Type 2 diabetes; Beijing China; China; Gut microbiota; Glucolipid metabolism; 16S rDNA sequencing; Ginsenoside Rb1; Salvianolic acid B
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.10598

To observe the effect of ginsenoside Rb1, salvianolic acid B and their combination on glucolipid metabolism and structural changes of gut microbiota. Eight-week-old C57BL/6J mice were fed 45% high-fat diet to induce obesity. The obese mice were randomly divided into four groups, Con group as model control, ginsenoside Rb1 (Rb1) group, salvianolic acid B (SalB) group and ginsenoside Rb1+ salvianolic acid B (Rb1SalB) group. Mice in Rb1, SalB and Rb1SalB group were treated by gavage with ginsenoside Rb1, salvianolic acid B and the combination of the two ingredients, respectively. While mice in Con group were given the same amount of sterile water. The intervention lasted 8 weeks. Body weight and fasting blood glucose were measured every 2 weeks. Oral glucose tolerance test was conducted on the 4th and 8th week of drug intervention. At the end of the experiment, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol and non-esterified fatty acid content as well as glycated hemoglobin were measured and feces were collected for 16S rDNA sequencing. Both ginsenoside Rb1 and Rb1SalB combination decreased body weight significantly (  < 0.05). Ginsenoside Rb1, salvianolic acid B and their combination alleviated fasting blood glucose, glycated hemoglobin and blood lipid profiles effectively (  < 0.05, compared with the corresponding indicators in Con group). Oral glucose tolerance test results at the 8th week showed that glucose tolerance was significantly improved in all three treatment groups. Ginsenoside Rb1, salvianolic acid B and their combination reduced the overall diversity of gut microbiota in feces and changed the microbial composition of the obese mice. LDA effect size (LefSe) analysis revealed the key indicator taxa corresponding to the treatment. Ginsenoside Rb1, salvianolic acid B and their combination could lower blood glucose and lipid level, and improve glucose tolerance of obese mice. The above effect may be at least partially through modulation of gut microbial composition.