Identification and characterization of core sludge and biofilm microbiota in anaerobic membrane bioreactors

• Published in: Environment international Vol. 133; no. Pt A; p. 105165
• Main Authors: Cheng, Hong, Cheng, Dan, Mao, Junwen, Lu, Ting, Hong, Pei-Ying
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.12.2019; Elsevier
• Subjects: Acinetobacter; Anaerobiosis; Bacteria - classification; biofilm; Biofilms; Bioreactors - microbiology; community structure; Core microorganisms; Correlative interaction; membrane bioreactors; Methanobacterium; microbial communities; Microbial Consortia; Microbiota; microorganisms; multivariate analysis; Net growth rate; Neutral assembly; Sewage - microbiology; sludge; Waste Disposal, Fluid; Correlative interaction; Neutral assembly; Core microorganisms; Net growth rate
• ISSN: 0160-4120; 1873-6750; 1873-6750
• DOI: 10.1016/j.envint.2019.105165

An analysis of sludge (i.e., 63 samples) and biofilm (i.e., 79 samples) sampled from 13 anaerobic membrane bioreactors (AnMBR) was conducted. Predominant microbial community identification and multivariate analysis indicate that these reactors showed different microbial community structure, but these differences had no impact on the overall AnMBR performance. Instead, core microbial genera which occurred in ≥90% of sludge (20 genera) and biofilm (12 genera) samples could potentially account for the AnMBR performance. A further calculation on net growth rate (NGR) of core genera in sludge suggested distribution into two main groups (i.e., I: low relative abundance and NGR, II: high relative abundance or high NGR). Consistent positive correlations between bacterial genera were observed among those that exhibited either high relative abundance or high NGR. The anaerobic microbial consortium in both sludge and biofilm were largely affected by stochastic dispersal and migration processes (i.e., neutral assembly). However, Acinetobacter spp. and Methanobacterium spp. occurred consistently in higher frequency in the biofilm but in lower occurrence frequency in the AnMBR permeate. Findings from this study suggest first, specific core microorganisms exist in the sludge regardless of the operating conditions of the AnMBRs, and second, prevention of biofoulant layer on anaerobic membranes can be devised by minimizing attachment of microbes on surfaces in a non-selective manner. [Display omitted] •Core genera including 20 in sludge and 12 in biofilm were identified from all reactors.•Among core groups, higher diversity and redundancy in fermenters than methanogens and syntrophs.•Core methanogens and syntrophs had higher net growth rate or relative abundance than most core fermenters.•Consortium in both sludge and biofilm were largely affected by neutral assembly.•Acinetobacter and Methanobacterium in biofilm deviated from neutral assembly.