Arsenic and mercury tolerant rhizobacteria that can improve phytoremediation of heavy metal contaminated soils

• Published in: PeerJ (San Francisco, CA) Vol. 11; p. e14697
• Main Authors: Rojas-Solis, Daniel, Larsen, John, Lindig-Cisneros, Roberto
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 12.01.2023; PeerJ, Inc; PeerJ Inc
• Subjects: Agricultural land; Agricultural wastes; Alphaproteobacteria; Arsenic; Auxin; Bacteria; Biodegradation, Environmental; Biofilms; Biotechnology; Chemical elements; Deposits; Ecology; Environmental conditions; Environmental Contamination and Remediation; Environmental quality; Fatty acids; Heavy metals; Indoleacetic acid; Mercuric chloride; Mercury; Metals, Heavy; Microbiology; Microorganisms; Mine tailings; Mining; Morphology; Phosphates; Phytoremediation; Plant growth; Remediation; Restoration; Rhizosphere; Rhizospheric bacteria; Siderophores; Soil; Soil contamination; Soil microbiology; Soil microorganisms; Soil pollution; Soil Science; Soils; Solubilization; Mexico; Mine tailings; Remediation; Restoration; Heavy metals; Rhizospheric bacteria
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.14697

Mining deposits often contain high levels of toxic elements such as mercury (Hg) and arsenic (As) representing strong environmental hazards. The purpose of this study was the isolation for plant growth promoting bacteria (PGPBs) that can improve phytoremediation of such mine waste deposits. We isolated native soil bacteria from the rhizosphere of plants of mine waste deposits and agricultural land that was previously mine tailings from Tlalpujahua Michoacán, Mexico, and were identified by their fatty acid profile according to the MIDI Sherlock system. Plant growth promoting traits of all bacterial isolates were examined including production of 3-indoleacetic acid (IAA), siderophores, biofilm formation, and phosphate solubilization. Finally, the response of selected bacteria to mercury and arsenic was examined an assay. A total 99 bacterial strains were isolated and 48 identified, representing 34 species belonging to 23 genera. Sixty six percent of the isolates produced IAA of which TL97 produced the most. TL36 performed best in terms of phosphate solubilization and production of siderophores. In terms of biofilm formation, TL76 was the best. Most of the bacteria isolates showed high level of tolerance to the arsenic (as HAsNa O and AsNaO ), whereas most isolates were susceptible to HgCl . Three of the selected bacteria with PGP traits TL36, TL49 and TL52 were also tolerant to high concentrations of mercury chloride, this might could be used for restoring or phytoremediating the adverse environmental conditions present in mine waste deposits.