Genetic analysis of DNA methylation in dyslipidemia: a case-control study

• Published in: PeerJ (San Francisco, CA) Vol. 10; p. e14590
• Main Authors: Liu, Shuai, Li, Yang, Wei, Xian, Adi, Dilare, Wang, Yong-Tao, Han, Min, Liu, Fen, Chen, Bang-Dang, Li, Xiao-Mei, Yang, Yi-Ning, Fu, Zhen-Yan, Ma, Yi-Tong
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 19.12.2022; PeerJ, Inc; PeerJ Inc
• Subjects: Age; Analysis; Cardiology; Cardiovascular disease; Cardiovascular diseases; Case-Control Studies; Cdc4 protein; Cholesterol; Coronary artery disease; Coronary Disease - genetics; CpG; CpG islands; Diabetes; Diabetes and Endocrinology; DNA; DNA methylation; DNA Methylation - genetics; DNA sequencing; Dyslipidemia; Dyslipidemias - genetics; Epigenetics; Gender; Gene expression; Gene regulation; Genes; Genetic analysis; Genetic aspects; Genetic diversity; Genetic research; GRINA; Haplotype; Haplotypes; Haplotypes - genetics; Heart diseases; Heart failure; Hematology; High density lipoprotein; Humans; Hyperlipidemia; Hypertension; Lipid metabolism; Lipids; Lipoproteins; Medical Genetics; Medical research; Medicine, Experimental; Metabolic disorders; Methylation; Molecular Biology; Mortality; Next-generation sequencing; Obesity; Physiological aspects; Statistical analysis; Triglycerides; United States > US; DNA methylation; CpG; Coronary artery disease; Haplotype; Dyslipidemia; GRINA
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.14590

Coronary heart disease has become the leading cause of death in developed countries, and dyslipidemia is closely associated with the risk of cardiovascular disease. Dyslipidemia is caused by the abnormal regulation of several genes and signaling pathways, and dyslipidemia is influenced mainly by genetic variation. , and genes are associated with lipid metabolism. In a recent GWAS study, the gene has been reported to be associated with dyslipidemia, but its molecular mechanism has not been thoroughly investigated. The correlation between the DNA methylation of these genes and lipid metabolism has not been studied. This study aimed to examine the relationship between the DNA methylation of these genes and the risk of dyslipidemia by comparing the methylation levels of dyslipidemia and control samples. A case-control research method was used in this study. The patient's blood samples were collected at the Heart Center of the First Affiliated Hospital of Xinjiang Medical University. In the Xinjiang Han population, 100 cases of hyperlipidemia and 80 cases of the control group were selected. The two groups were age and gender-matched. Quantitative methylation analysis of CpG sites in the gene promoter regions of six genes was performed by Solexa high-throughput sequencing. The DNA methylation levels of 23 CpG sites in six genes were shown to be associated with hyperlipidemia, and a total of 20 DNA methylation haplotypes showed statistically significant differences between the two groups. When compared with the control group, the dyslipidemia group had significantly higher levels of methylation in the gene (2.68 2.36, = 0.04). Additionally, we also discovered a significant methylation haplotype of GRINA ( = 0.017). The findings of this study reveal that the DNA methylation of increases the risk for dyslipidemia in humans.