Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques

• Published in: PeerJ (San Francisco, CA) Vol. 9; p. e12596
• Main Authors: Metwally, Dina M, Alajmi, Reem, Alsulami, Muslimah N, Al-Turaiki, Isra M, Abdel-Gaber, Rewaida, Alkhuriji, Afrah F, Albohiri, Haleema H, Mohamed, Khalil, Baghdadi, Hanadi B, El-Khadragy, Manal F, Isaias, Guillermo T, El-Ashram, Saeed
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 10.12.2021; PeerJ, Inc; PeerJ Inc
• Subjects: 18S rRNA; Animals; Bioinformatics; Ethylenediaminetetraacetic acid; Females; Goats; Infections; Methods; Parasites; Parasitology; Phylogenetics; Phylogeny; Polymerase chain reaction; Polymerase chain reaction (PCR); RNA; rRNA 18S; Sheep; Theileria; Theileria spp; Veterinary Medicine; Zoology; Saudi Arabia; United Kingdom > UK; Iran; United States > US; Polymerase chain reaction (PCR); 18S rRNA; Theileria spp
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.12596

Thileriosis is a tick -born disease caused by hemoprotozoan parasites which has global veterinary and economic implications. Blood samples were collected from 216 sheep and 83 goats from Jeddah, Saudi Arabia, were analyzed to determine whether the animals were infected with spp. parasites. The parasites were detected using a polymerase chain reaction (PCR) targeting the gene of followed by sequencing. According to obtained findings, spp. were detected in sheep (57.8%, 48/83) and goats (51.9%, 112/216). Phylogenetic analysis to sequence data showed that identified in this study were found to be closely connected to an isolate from Turkey, with 84.4-99.8% pairwise identity and 52.35-99.79% coverage.