Stimulation of Diethylnitrosamine Metabolism Reduces Its General Toxic and Hepatocarcinogenic Effects

• Published in: Bulletin of experimental biology and medicine Vol. 162; no. 1; pp. 98 - 101
• Main Authors: Il’nitskaya, S. I., Kaledin, V. I., Bogdanova, L. A., Morozkova, T. S., Kapustina, V. I., Perepechaeva, M. L., Grishanova, A. Yu
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2016; Springer; Springer Nature B.V
• Subjects: Animals; Animals, Suckling; Benzene; Biomedical and Life Sciences; Biomedicine; Body weight; Body Weight - drug effects; Body weight loss; Carcinogenesis - drug effects; Carcinogenesis - metabolism; Carcinogenesis - pathology; Cell Biology; Cytochrome P-450 CYP2E1 - metabolism; Cytochrome P-450 Enzyme Inducers - pharmacology; Cytochrome P450; Diethylnitrosamine; Diethylnitrosamine - metabolism; Diethylnitrosamine - toxicity; Food consumption; House mouse; Inactivation, Metabolic - drug effects; Injection; Internal Medicine; Laboratory Medicine; Liver; Liver - drug effects; Liver - enzymology; Liver Neoplasms, Experimental - chemically induced; Liver Neoplasms, Experimental - drug therapy; Liver Neoplasms, Experimental - enzymology; Liver Neoplasms, Experimental - pathology; Male; Metabolism; Mice; Mice, Inbred C57BL; Mice, Inbred ICR; Microsomes; Microsomes, Liver - drug effects; Microsomes, Liver - enzymology; Nitrophenols - metabolism; Nodes; p-Nitrophenol; Pathology; Physiological aspects; Pyridines - pharmacology; Suckling behavior; Tumor Burden - drug effects; Tumors; toxicity; diethylnitrosamine; cytochrome P450 2e1; hepatocarcinogenesis
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-016-3555-3

The general toxic and hepatocarcinogenic effects of diethylnitrosamine after stimulation of its metabolism with 1,4-bis[2-(3,5-dichloropyridyloxy)]-benzene (TCPOBOP) were studied. The hydroxylating activity of liver microsomes of C57Bl/6Mv mice towards p-nitrophenol increased more than 4-fold 3 days after injection of TCPOBOP. Injection of diethylnitrosamine 3 days after TCPOBOP caused a lesser body weight loss and decrease of food consumption in C57Bl/6Mv mice than in response to diethylnitrosamine without preinduction. Injection of diethylnitrosamine to suckling ICR mice after TCPOBOP induction of cytochrome P450 2e1 activity led to development of 2-fold lesser number of tumors and pretumorous nodes in the liver in comparison with animals injected with diethylnitrosamine without induction. These data indicated that metabolism stimulation reduced the general toxic and hepatocarcinogenic effects of diethylnitrosamine.