A comparative study of bacterial diversity based on effects of three different shade shed types in the rhizosphere of Panax quiquefolium L

• Published in: PeerJ (San Francisco, CA) Vol. 10; p. e12807
• Main Authors: Wang, Xianchang, Guo, Xu, Hou, Lijuan, Zhang, Jiaohong, Hu, Jing, Zhang, Feng, Mao, Jilei, Wang, Zhifen, Zhang, Congjing, Han, Jinlong, Zhu, Yanwei, Liu, Chao, Sun, Jinyue, Shan, Chenggang
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 09.02.2022; PeerJ, Inc; PeerJ Inc
• Subjects: 16s rRNA; Agricultural Science; American ginseng; Bacteria; Bacteria - genetics; Bar codes; Ginseng; Microbiology; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Molecular Biology; Panax - genetics; Permeability; Plant diseases; Plant Roots - microbiology; Plant Science; Rhizosphere; Rhizosphere microorganisms; RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Shade; Shade shed; Shading; Software; Soil; Soil - chemistry; Soil Microbiology; Taxonomy; Variance analysis; China; United States > US; Soil; Rhizosphere; Bacteria; 16s rRNA; American ginseng; Shade shed
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.12807

Shading is an important factor affecting the cultivation of American ginseng, as it influences crop quality and yield. Rhizosphere microorganisms are also crucial for normal plant growth and development. However, whether different shade types significantly change American ginseng rhizosphere microorganisms is unknown. This study evaluated the rhizosphere soils of American ginseng under traditional, high flag and high arch shade sheds. High-throughput 16S rRNA gene sequencing determined the change of rhizosphere bacterial communities. The microbial diversity in rhizosphere soils of American ginseng significantly changed under different shading conditions. The bacteria diversity was more abundant in the high arch shade than flat and traditional shades. Different bacterial genera, including , , , and , showed significantly different abundances. Different shading conditions changed the microbial metabolic function in the American ginseng rhizosphere soils. The three types of shade sheds had specific enriched functional groups. The abundance of ATP-binding cassette (ABC) transporters consistently increased in the bacterial microbiota. These results help understand the influence of shading systems on the rhizosphere microecology of American ginseng, and contribute to the American ginseng cultivation.