Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila

PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is r...

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Published inGenes & development Vol. 24; no. 22; pp. 2493 - 2498
Main Authors Saito, Kuniaki, Ishizu, Hirotsugu, Komai, Miharu, Kotani, Hazuki, Kawamura, Yoshinori, Nishida, Kazumichi M, Siomi, Haruhiko, Siomi, Mikiko C
Format Journal Article
LanguageEnglish
Published United States Cold Spring Harbor Laboratory Press 15.11.2010
Subjects
Animals
Cell Line
Cell Nucleus - metabolism
Cytoplasm - metabolism
DNA Transposable Elements - physiology
Drosophila
Drosophila melanogaster - metabolism
Drosophila melanogaster - physiology
Drosophila Proteins - metabolism
Endoribonucleases - metabolism
Protein Transport
Research Communication
RNA Helicases - metabolism
RNA, Small Interfering - biosynthesis
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Summary:PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing.
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These authors contributed equally to this work.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1989510