Spontaneous rate of sister chromatid exchanges (SCEs) and BrdU dose-response relationships in mitotic chromosomes of goat (Capra hircus L.)

• Published in: Hereditas Vol. 124; no. 2; pp. 137 - 144
• Main Authors: di Berardino, Dino, Jovino, Valeria, Lioi, Maria Brigida, Scarfi, Maria Rosaria, Burguete, Isabel
• Format: Journal Article
• Language: English
• Published: Oxford, UK Munksgaard International Publishers 01.01.1996
• Subjects: Animals; Bromodeoxyuridine; Capra hircus; Chromosomes; Dose-Response Relationship, Drug; Goats - genetics; Mitosis - drug effects; Sister Chromatid Exchange
• ISSN: 0018-0661; 1601-5223
• DOI: 10.1111/j.1601-5223.1996.t01-1-00137.x

The spontaneous level of sister chromatid exchange (SCEs) in the goat, estimated by exposing peripheral blood lymphocytes to 0.1 μg/ml of 5‐bromodeoxyuridine (BrdU), was 3.28 ± 1.71 SCE/cell, 1.64 SCE/cell generation and 0.027 SCE/chromosome. The dose–response curve of SCE/cell, observed by exposing the cells to 0.1, 0.25, 0.5, 1.0, 2.5, and 5.0 μg/ml of BrdU, rose rapidly from 0.1 to 0.5 μg/ml, remained fairly stable from 0.5 to 1.0 μg/ml and rose less rapidly from 1.0 to 5.0 μg/ml of BrdU. The frequency distribution of sister chromatid exchanges/cell and that of chromosomes showing various number of exchanges followed the Poisson probability at all BrdU levels; only at 5.0 μg/ml of BrdU was the fit found on the border of the 5% probability level. The usefulness of determining the spontaneous level of SCE/cell in domestic animals is discussed in relation to its possible application for a more precise evaluation of the genotoxic effects of environmental pollutants.