Anti-allergic Inflammatory Components from the Leaves of Piper crocatum Ruiz & Pav

• Published in: Biological & pharmaceutical bulletin Vol. 44; no. 2; pp. 245 - 250
• Main Authors: Gong, Yue, Li, Hong Xu, Guo, Rui Hong, Widowati, Wahyu, Kim, Young Ho, Yang, Seo Young, Kim, Young Ran
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 01.02.2021; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: allergic inflammation; Antibacterial activity; Antioxidants; Antitumor activity; Bovine serum albumin; catechaldehyde; Cytotoxicity; Immune response; Immunoglobulin E; inducible nitric oxide synthase; Inflammation; Leaves; Lipopolysaccharides; Macrophages; Medicinal plants; Methanol; Nitric oxide; Nitric-oxide synthase; NMR; Nuclear magnetic resonance; Piper crocatum Ruiz & Pav; β-hexosaminidase; Piper crocatum Ruiz & Pav; allergic inflammation; nitric oxide; β-hexosaminidase; catechaldehyde; inducible nitric oxide synthase
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.b20-00726

Piper crocatum Ruiz & Pav. (P. crocatum), a traditional medicinal plant, has been shown to possess various pharmacological activities, including anticancer activity, antioxidant activity, antibacterial activity, anti-hyperglycemic activity, anti-allergic inflammatory activity and others. To identify the potential anti-allergic inflammatory effective constituents of P. crocatum, 13 single compounds were isolated from the methanol extract of P. crocatum leaves, and their structures were identified by contrasting their NMR spectroscopic data and previously published papers. First, the anti-allergic inflammatory activities of these single compounds were examined by accessing immune function related biomarkers such as nitric oxide (NO) and β-hexosaminidase. We found that the methanol extract and catechaldehyde (compound 1) potently suppressed NO production. Additionally, Western blot analysis showed that P. crocatum methanol extract and compound 1 suppressed the production of NO by reducing inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Consistent with these observations, P. crocatum methanol extract and compound 1 remarkably decreased β-hexosaminidase release from RBL-2H3 cells stimulated with 2,4-dinitrophenylated bovine serum albumin (DNP-BSA)-specific immunoglobulin E (IgE) antibodies. Furthermore, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay indicated that P. crocatum methanol extract and compound 1 exhibited no cytotoxicity to RAW264.7 and RBL-2H3 cells. Based on these findings, compound 1 is suggested as an active anti-allergic inflammatory component of P. crocatum.