First report of human astrovirus MLB2 in Brazil detected in feces of children with acute gastroenteritis living in the state of Roraima, Northern Brazil

• Published in: The Brazilian journal of infectious diseases Vol. 24; no. 6; pp. 575 - 579
• Main Authors: Olivares, Alberto Ignacio Olivares, Moraes, Marcia Terezinha Baroni de, Queiroz, Herika Caroline Fernandes de, Pimenta, Yan Cardoso, Leite, José Paulo Gagliardi
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Elsevier España, S.L.U 01.11.2020; Contexto; Elsevier; Brazilian Society of Infectious Diseases
• Subjects: Acute gastroenteritis; Amazon; Astroviridae Infections - diagnosis; Astroviridae Infections - epidemiology; Brazil; Brief Communication; Care and treatment; Child; Children; Diagnosis; Diseases; Feces; Gastroenteritis; Gastroenteritis - diagnosis; Genetic aspects; Genotype; Genotypes; Health aspects; Human astrovirus; Humans; Identification and classification; Infant; INFECTIOUS DISEASES; Mamastrovirus - genetics; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; RNA viruses; Brazil; Human astrovirus; Amazon; Genotypes; Acute gastroenteritis
• ISSN: 1413-8670; 1678-4391; 1678-4391
• DOI: 10.1016/j.bjid.2020.10.003

Human astrovirus (HAstV) 1–8 and highly divergent HAstVMLB1−3 genotypes have been detected in children both with and without acute gastroenteritis (AGE). One hundred and seventy fecal samples from children (≤5 years old) living in the Amazon region were evaluated for the presence of HAstV1–8, HAstV MLB1−3 and HAstVVA1−3, using an usual RT-PCR protocol and a new protocol with specific primers designed to detect HAstVMLB1−3. HAstVMLB1 and HAstV MLB2, as well as the HAstV3 and 5 genotypes were detected. HAstVMLB1−2 genotype was detected for the first time in Brazil at a frequency of 3.5% (6/170).