尼龙筛绢基质对对虾养殖水体细菌群落结构演替的影响
采用基于16S rDNA的PCR-DGGE(变性梯度凝胶电泳)技术,比较分析了添加尼龙筛绢网基质对凡纳滨对虾(Litopenaeus vannamei)精养系统中水体细菌群落结构演替的影响。结果表明:1)基质组水体菌群结构丰富度稍低于对照组;2)基质组水体菌群结构相似性始终高于对照组;随着时间的推移,基质组的相似性系数不断升高,而对照组出现了先升高后下降的波动;3)对照组水体养殖前期优势菌主要为变形菌门和未培养细菌,在养殖后期则以厚壁菌门芽孢杆菌属(Bacillus spp.)细菌为主,基质组水体菌群则在整个养殖周期中均以厚壁菌门芽孢杆菌属细菌和未培养细菌为主。该研究结果可为进一步阐明人工基质...
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Published in | 南方水产科学 Vol. 12; no. 1; pp. 17 - 22 |
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Main Author | |
Format | Journal Article |
Language | Chinese |
Published |
中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东 广州 510300%中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东 广州 510300
2016
上海海洋大学水产与生命学院,上海 201306 |
Subjects | |
Online Access | Get full text |
ISSN | 2095-0780 |
DOI | 10.3969/j.issn.2095-0780.2016.01.003 |
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Summary: | 采用基于16S rDNA的PCR-DGGE(变性梯度凝胶电泳)技术,比较分析了添加尼龙筛绢网基质对凡纳滨对虾(Litopenaeus vannamei)精养系统中水体细菌群落结构演替的影响。结果表明:1)基质组水体菌群结构丰富度稍低于对照组;2)基质组水体菌群结构相似性始终高于对照组;随着时间的推移,基质组的相似性系数不断升高,而对照组出现了先升高后下降的波动;3)对照组水体养殖前期优势菌主要为变形菌门和未培养细菌,在养殖后期则以厚壁菌门芽孢杆菌属(Bacillus spp.)细菌为主,基质组水体菌群则在整个养殖周期中均以厚壁菌门芽孢杆菌属细菌和未培养细菌为主。该研究结果可为进一步阐明人工基质养殖系统中微生物的生态调控功能和作用机制提供参考。 |
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Bibliography: | shrimp; artificial substrate; bacterial community; DGGE 44-1683/S DONG Hongbiao;CHEN Liangliang;FENG Zhenhua;WEN Guoliang;DUAN Yafei;LI Zhuojia;ZHANG Jiasong ( 1. Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture ; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2. College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China) The bacterial community composition in the substrate-biofilm shrimp culture system was monitored and investigated by pcrdenaturing gradient gel electrophoresis( DGGE). Results show that: 1) The water flora structure of substrate group was less diversified than that of the control. 2) The similarity coefficient of substrate group increased with time,while that of the control increased first then decreased. 3) Uncultured Firmicutes( Bacillus spp.),the dominant bacteria at the beginning of culture in the control,were uncultured Proteobacteria and uncultured bacterium, |
ISSN: | 2095-0780 |
DOI: | 10.3969/j.issn.2095-0780.2016.01.003 |