Novel magnetic bovine serum albumin imprinted polymers with a matrix of carbon nanotubes, and their application to protein separation

• Published in: Analytical and bioanalytical chemistry Vol. 401; no. 9; pp. 2855 - 2863
• Main Authors: Zhang, Zhaohui, Yang, Xiao, Chen, Xing, Zhang, Minlei, Luo, Lijuan, Peng, Mijun, Yao, Shouzhuo
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.11.2011; Springer
• Subjects: Adsorption; Albumin; Analytical Chemistry; Animals; Biochemistry; Cattle; Characterization and Evaluation of Materials; Chemical Fractionation - methods; Chemistry; Chemistry and Materials Science; Exact sciences and technology; Ferric Compounds - chemistry; Food Science; Imprinted polymers; Infrared spectroscopy; Laboratory Medicine; Magnetic Fields; Magnetics; Microscopy, Electron, Scanning; Molecular Imprinting - methods; Monitoring/Environmental Analysis; Nanostructure; Nanotechnology - methods; Nanotubes; Nanotubes, Carbon - chemistry; Nanotubes, Carbon - ultrastructure; Original Paper; Particle Size; Polymers; Polymers - chemistry; Polymers - metabolism; Proteins; Recognition; Scanning electron microscopy; Separation; Serum albumin; Serum Albumin, Bovine - chemistry; Serum Albumin, Bovine - isolation & purification; Serum Albumin, Bovine - metabolism; Solid Phase Extraction; Spectrometric and optical methods; Spectroscopy, Fourier Transform Infrared; Carbon nanotube; Protein separation; Molecularly imprinted polymers (MIPs); Surface imprinting; Magnetic nanoparticles; Solid phase extraction; Fourier transform spectroscopy; Molecular structure; Nanoparticle; Carbon nanotubes; Selectivity; Time; Chemical enrichment; Magnetic material; Serum; Magnetic field; Ungulata; Scanning electron microscopy; Purification; Bovine; Sample; Use; Polymer; Serum albumin; Protein; Thickness; Sorption; Infrared spectrometry; Vertebrata; Mammalia; Adsorption; Feasibility; Artiodactyla; Application; Molecular imprinting
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-011-5373-9

Novel magnetic multi-walled carbon nanotubes@Fe 3 O 4 molecularly imprinted polymers (MWNTs@Fe 3 O 4 -MIPs) intended for bovine serum albumin (BSA) recognition were successfully developed. The MWNTs@Fe 3 O 4 -MIPs were characterized with scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). Scanning electron microscopy images showed that the Fe 3 O 4 nanoparticles (diameter: 50–60 nm) were coated with a layer of MIPs with an average thickness of 25–30 nm. The magnetic material was easily dispersed and retrieved through the application of an external magnetic field. Adsorption experiments showed that the estimated maximum amount of BSA that could be adsorbed onto the MWNTs@Fe 3 O 4 -MIPs was 52.8 mg/g, and the time taken to reach equilibrium was about 40 min. Meanwhile, the MWNTs@Fe 3 O 4 -MIPs exhibited excellent selectivity towards (i.e., recognition of) BSA. The feasibility of the use of the MWNTs@Fe 3 O 4 -MIPs as a solid-phase extraction (SPE) sorbent was evaluated, and the results showed that the MWNTs@Fe 3 O 4 -MIPs were able to separate the template protein BSA from a binary protein solution. The proposed sorbent based on MWNTs@Fe 3 O 4 -MIPs for BSA separation exhibited satisfactory recoveries ranging from 92.0% to 97.3% in real samples. It was also successfully used for the purification of BSA from bovine calf serum. Figure Procedure for preparing magnetic protein imprinted polymers.