Fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates
Two step, fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates. We have developed a fluorescence technique for the detection of Hg2+ and Pb2+ ions using polythymine (T33)/benzothiazolium-4-quinolinium dimer derivative (TOTO-3) and polyguanine (G33)/terbium ions (...
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Published in | Talanta (Oxford) Vol. 84; no. 2; pp. 324 - 329 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
15.04.2011
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Two step, fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates.
We have developed a fluorescence technique for the detection of Hg2+ and Pb2+ ions using polythymine (T33)/benzothiazolium-4-quinolinium dimer derivative (TOTO-3) and polyguanine (G33)/terbium ions (Tb3+) conjugates, respectively. Hg2+ ions induce T33 to form folded structures, leading to increased fluorescence of the T33/TOTO-3 conjugates. Because Pb2+ ions compete with Tb3+ ions to form complexes with G33, the extent of formation of the G33–Tb3+ complexes decreases upon increasing the Pb2+ concentration, leading to decreased fluorescence at 545nm when excited at 290nm. To minimize interference from Hg2+ ions during the detection of Pb2+ ions, we conducted two-step fluorescence measurements; prior to addition of the G33/Tb3+ probe, we recorded the fluorescence of a mixture of the T33/TOTO-3 conjugates and Hg2+ ions. The fluorescence signal obtained was linear with respect to the Hg2+ concentration over the range 25.0–500nM (R2=0.99); for Pb2+ ions, it was linear over the range 3.0–50nM (R2=0.98). The limits of detection (at a signal-to-noise ratio of 3) for Hg2+ and Pb2+ ions were 10.0 and 1.0nM, respectively. Relative to other techniques for the detection of Hg2+ and Pb2+ ions in soil and water samples, our present approach is simpler, faster, and more cost-effective. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0039-9140 1873-3573 |
DOI: | 10.1016/j.talanta.2011.01.016 |