Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles

• Published in: Molecular and cellular endocrinology Vol. 332; no. 1-2; pp. 189 - 195
• Main Authors: Brogan, Rebecca S., MacGibeny, Margaret, Mix, Scott, Thompson, Christopher, Puttabyatappa, Muraly, VandeVoort, Catherine A., Chaffin, Charles L.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 30.01.2011
• Subjects: Animals; Body Fluids - chemistry; Cell Line; Cellular; Chorionic Gonadotropin - pharmacology; Dynamics; embryogenesis; Embryos; Excitatory Amino Acid Transporter 2 - genetics; Excitatory Amino Acid Transporter 2 - metabolism; Female; follicular fluid; Glucose; Glucose - metabolism; glucose-6-phosphate 1-dehydrogenase; glycogen; Glycolysis; Granulosa cells; Granulosa Cells - cytology; Granulosa Cells - drug effects; Granulosa Cells - physiology; hexokinase; human chorionic gonadotropin; Humans; Hyperglycemia; Insulin - pharmacology; liver; Luteinization; Luteinization - physiology; Macaca - anatomy & histology; Macaca - physiology; Macaque; messenger RNA; Murals; oocytes; Oocytes - cytology; Oocytes - physiology; Ovarian Follicle - cytology; Ovarian Follicle - drug effects; Ovarian Follicle - metabolism; phosphorylase; Phosphorylases; Physiology; Primates; pyruvic acid; Stimulation; Granulosa cells; Glycolysis; Macaque; Glucose; Luteinization
• ISSN: 0303-7207; 1872-8057
• DOI: 10.1016/j.mce.2010.10.011

Glucose is important to the maturation of the oocyte and development of the embryo, while hyperglycemia results in profound reproductive and developmental consequences. However, the normal physiology of glucose in the ovary remains poorly understood. The goal of this study was to determine intra-follicular glucose dynamics during the periovulatory interval in non-human primates undergoing controlled ovarian stimulation protocols. Follicular fluid and mural granulosa cells were isolated before or up to 24h after an ovulatory hCG bolus, and the human granulosa-lutein cell line hGL5 was used. Intra-follicular glucose increased 3h after hCG, and remained at that level until 12h when levels decline back to pre-hCG concentrations. Pyruvate and lactate concentrations in the follicle were not strongly altered by hCG. Mural granulosa cell expression of hexokinase 1 and 2, and glucose-6-phosphate dehydrogenase mRNA decreased following hCG, while glycogen phosphorylase (liver form) increased following hCG. Glucose uptake by hGL5 cells was delayed until 24h following stimulation. In summary, intra-follicular glucose increases following an ovulatory stimulus and mural granulosa cells do not appear able to utilize it, sparing the glucose for the cumulus-oocyte complex.