Genetic Comparison of Brucella canis Isolates by the MLVA Assay in South Korea

• Published in: Journal of Veterinary Medical Science Vol. 73; no. 6; pp. 779 - 786
• Main Authors: KANG, Sung-Il, HEO, Eun Jeong, CHO, Donghee, KIM, Jong Wan, KIM, Ji-Yeon, JUNG, Suk Chan, HER, Moon
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2011; Japan Science and Technology Agency
• Subjects: Animals; Brucella; Brucella canis; Brucella canis - genetics; Brucella canis - isolation & purification; Brucellosis - epidemiology; Brucellosis - microbiology; Brucellosis - veterinary; Canis; countermeasure; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Dog Diseases - epidemiology; Dog Diseases - microbiology; Dogs; epidemiological tool; Genetic Variation; genotyping; Minisatellite Repeats - genetics; MLVA; Phylogeny; Polymerase Chain Reaction - veterinary; Republic of Korea - epidemiology; Republic of Korea
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.10-0334

The multiple-locus VNTR analysis (MLVA) assay is a method frequently employed as a molecular epidemiological tool for Brucella genetic fingerprinting. The purpose of this study was to assess the genotyping of 77 B. canis isolates from 14 different dog breeding farms in Korea by the MLVA assay and to compare the epidemiological relationships between the Korean isolates and foreign ones. Simpson's diversity index for 17 loci showed a range from 0 to 0.846 in 77 B. canis isolates. B. canis isolates in Korea were observed to have high genetic diversity at the most variable loci and were divided into 30 distinct genotypes by phylogenetic analysis. Some B. canis isolates were closely related to previously typed isolates in other countries. The MLVA assay can be helpful to analyze the epidemiological correlation of B. canis isolates in domestic pet animals and to track the geographic origin by comparing the genetic patterns with foreign isolates. Therefore, the MLVA assay will be useful as a tool for control and preventive measures of canine brucellosis.