A minimal view of single-particle imaging with X-ray lasers
The ability to serially interrogate single biomolecules with femtosecond X-ray pulses from free-electron lasers has ushered in the possibility of determining the three-dimensional structure of biomolecules without crystallization. However, the complexity of imaging a sample's structure from ver...
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Published in | Philosophical transactions of the Royal Society of London. Series B. Biological sciences Vol. 369; no. 1647; p. 20130328 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
England
The Royal Society
17.07.2014
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Subjects | |
Online Access | Get full text |
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Summary: | The ability to serially interrogate single biomolecules with femtosecond X-ray pulses from free-electron lasers has ushered in the possibility of determining the three-dimensional structure of biomolecules without crystallization. However, the complexity of imaging a sample's structure from very many of its noisy and incomplete diffraction data can be daunting. In this review, we introduce a simple analogue of this imaging workflow, use it to describe a structure reconstruction algorithm based on the expectation maximization principle, and consider the effects of extraneous noise. Such a minimal model can aid experiment and algorithm design in future studies. |
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Bibliography: | istex:375D42C9BEAC716713D8512549E897702CCCF2C0 One contribution of 27 to a Discussion Meeting Issue ‘Biology with free-electron X-ray lasers’. ark:/67375/V84-9DQWZH73-3 href:rstb20130328.pdf ArticleID:rstb20130328 Discussion Meeting Issue 'Biology with free-electron X-ray lasers' organized and edited by John C. H. Spence and Henry N. Chapman ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
ISSN: | 0962-8436 1471-2970 |
DOI: | 10.1098/rstb.2013.0328 |