Chitinase Production by Streptomyces sp. ANU 6277

• Published in: Brazilian journal of microbiology Vol. 40; no. 4; pp. 725 - 733
• Main Authors: Narayana, Kolla J.P.(Acharya Nagarjuna University Department of Microbiology), Vijayalakshmi, Muvva(Acharya Nagarjuna University Department of Microbiology)
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Sociedade Brasileira de Microbiologia 01.10.2009; Springer Nature B.V
• Subjects: Bacteria; Biotechnology; Characterization; Chitinase; Enzymes; Fermentation; Industrial Microbiology; MICROBIOLOGY; Optimization; Streptomyces sp. ANU 6277; Characterization; Chitinase; Streptomyces sp. ANU 6277; Optimization
• ISSN: 1517-8382; 1678-4405; 1678-4405; 1517-8382
• DOI: 10.1590/s1517-83822009000400002

Chitinase production by a terrestrial Streptomyces sp. ANU 6277 was studied under sub-merged fermentation. Chitinase production started after 24 h of incubation and reached maximum levels after 60 h of cultivation. A high level of chitinase activity was observed in the culture medium with pH 6 at 35ºC. Culture medium amended with 1% chitin was found to be suitable for maximum production of chitinase. An optimum concentration of colloidal chitin for chitinase production was determined. Studies on the influence of additional carbon and nitrogen sources on chitinase production revealed that starch and yeast extract served as good carbon and nitrogen sources to enhance chitinase yield.Chitinase was purified from crude enzyme extract by single step gel filtration by Sephadex G-100. Purified chitinase of the strain exhibited a distinct protein band near 45 kDa by means of SDS-PAGE.