Proliferation, differentiation and amyloid-β production in neural progenitor cells isolated from TgCRND8 mice

• Published in: Neuroscience Vol. 261; no. 100; pp. 52 - 59
• Main Authors: Kanemoto, S, Griffin, J, Markham-Coultes, K, Aubert, I, Tandon, A, George-Hyslop, P.S, Fraser, P.E
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 07.03.2014; Elsevier; Elsevier Science
• Subjects: Adult and adolescent clinical studies; Alzheimer Disease; Alzheimer’s disease; amyloid; Amyloid beta-Peptides - metabolism; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - metabolism; Amyloid Precursor Protein Secretases - metabolism; Animals; Biological and medical sciences; Cell Count; Cell Survival - physiology; Cells, Cultured; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Dentate Gyrus - physiology; DNA-Binding Proteins; Glial Fibrillary Acidic Protein; Hippocampus - physiology; Immunohistochemistry; Medical sciences; Mice; Mice, Transgenic; Nerve Tissue Proteins - metabolism; neural progenitor cells; Neural Stem Cells - physiology; Neurogenesis - physiology; Neuroglia - physiology; Neurology; Neurons - physiology; Nuclear Proteins - metabolism; Organic mental disorders. Neuropsychology; Psychology. Psychoanalysis. Psychiatry; Psychopathology. Psychiatry; S100 Calcium Binding Protein beta Subunit - metabolism; transgenic mice; APP; DMSO; HRP; SGZ; neuronal nuclear antigen; phosphate-buffered saline; enzyme-linked immunoabsorbent assay; 5-bromo-2-deoxyuridine; Aβ; DMEM; fibroblast growth factor; dimethyl sulfoxide; analysis of variance; transgenic mice; ANOVA; amyloid precursor protein; propidium iodide; FBS; SVZ; AICD; Non-Tg; βFGF; PBS; Dulbecco's modified Eagle medium; subventricular zone; AD; Alzheimer’s disease; EGF; amyloid-β; S.D; subgranular zone; neural progenitor cells; GFAP; fetal bovine serum; amyloid precursor protein intracellular domain; amyloid; NeuN; horse radish peroxidase; NPCs; PI; epidermal growth factor; glial fibrillary acidic protein; BrdU; ELISA; non-transgenic; standard deviation; Cell proliferation; Nervous system diseases; Alzheimer disease; Rodentia; Precursor cell; Transgenic animal; Cerebral disorder; Vertebrata; Mammalia; Mouse; β Amyloid protein; Central nervous system disease; Degenerative disease; Amyloid; Differentiation; Alzheimer's disease; Progenitor cell
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2013.12.021

Highlights • In young TgCRND8 mice, proliferation of newborn cells in dentate gyrus is increased, compared with Non-Tg control mice. • On the contrary, differentiation to neural progenitor cells of newborn cells in the TgCRND8 hippocampus is impaired. • Neurosphere cultures isolated from hippocampi of TgCRND8 mice show low viability than those from Non-Tg control. • Neurosphere cultures from TgCRND8 mice secrete high levels of Aβ peptide.