Anti-inflammatory activity of Jatropha curcas Linn. latex in cream formulation on CD68 expression in mice skin wound

• Published in: Veterinary World Vol. 11; no. 2; pp. 99 - 103
• Main Authors: Salim, Muhammad Nur, Masyitha, Dian, Harris, Abdul, Balqis, Ummu, Iskandar, Cut Dahlia, Hambal, Muhammad, Darmawi
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01.02.2018
• Subjects: anti-inflammatory; Anti-inflammatory agents; CD68; Cream; Inflammation; Jatropha curcas; Jatropha curcas latex cream; Latex; Macrophages; Mice; Procaine; Skin; Sulfadiazine; Surgery; Wound care; Wound healing; anti-inflammatory; wound healing; Jatropha curcas latex cream; CD68
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2018.99-103

The purpose of the present study was to determine the potential of latex in the cream formulation on CD68 immune expression (macrophages) during inflammatory phase wound healing process in mice skin. Amount of 12 two-months-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. The mice were allotted into four groups of each, entire surface of each group wound covered by base cream control, sulfadiazine 0.1% cream, latex cream 10% and, 15%, respectively. All experiments were performed twice a day for 3 days. The wound healing was assayed in stained histological sections in immunohistochemical of the wounds. CD68 expression was investigated under a microscope. The results showed that the cream from the 10% and 15% latex of revealed moderate immune reaction to CD68 on wound healing. We concluded that the latex cream of possesses anti-inflammatory activity in wound healing process of mice skin.