Increased cellulose production from sucrose by Acetobacter after introducing the sucrose phosphorylase gene

• Published in: Bioscience, biotechnology, and biochemistry Vol. 62; no. 9; pp. 1778 - 1780
• Main Authors: Tonouchi, N. (Bio-Polymer Research Co. Ltd., Kawasaki, Kanagawa (Japan)), Horinouchi, S, Tsuchida, T, Yoshinaga, F
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 1998; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ACETOBACTER; Biological and medical sciences; Biotechnology; CELLULOSE; CELULOSA; FOSFOPIROLASA; Fundamental and applied biological sciences. Psychology; GENE; gene introduction; GENES; Genetic engineering; Genetic technics; Leuconostoc mesenteroides; Methods. Procedures. Technologies; Modification of gene expression level; PHOSPHORYLASE; SACCHAROSE; SUCROSA; SUCROSE; sucrose phosphorylase; Acetobacteraceae; Recombinant microorganism; Genetic transformation; Sucrose; Enzyme; Transferases; Glycosyltransferases; Cellulose; Metabolism; Acetobacter; Sucrose phosphorylase; Production; Microorganism culture; Bacteria; Hexosyltransferases; cellulose; sucrose phosphorylase; gene introduction
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.62.1778

A sucrose phosphorylase (SPase) gene derived from Leuconostoc mesenteroides was introduced into a cellulose-producing acetobacter strain and expressed under the lac promoter. The activity of the SPase was detected in extracts of the transformed cells and cellulose production from sucrose by the cells was found to have increased, which strongly suggests that the increase was the result of the new metabolizing pathway. Furthermore, the level of SPase expression was increased by altering the leng