Development, identification, and characterization of a glyphosate-tolerant soybean line

Glyphosate (N-phosphonomethyl-glycine) is the active ingredient in the nonselective herbicide Roundup. The sensitivity of crop plants to glyphosate has limited its in-season use as a postemergence herbicide. The extension of the use of Roundup herbicide to allow in-season application in major crops...

Full description

Saved in:
Bibliographic Details
Published inCrop science Vol. 35; no. 5
Main Authors Padgette, S.R. (Monsanto Co., St. Louis, MO.), Kolacz, K.H, Delannay, X, Re, D.B, LaVallee, B.J, Tinius, C.N, Rhodes, W.K, Otero, Y.I, Barry, G.F, Eichholtz, D.A
Format Journal Article
LanguageEnglish
Published 01.09.1995
Subjects
AGROBACTERIUM TUMEFACIENS
EXPRESION GENICA
EXPRESSION DES GENES
GENE
GENES
GENETICA
GENETIQUE
GLIFOSATO
GLYCINE MAX
GLYPHOSATE
HERBICIDAS
HERBICIDE
HEREDITE
HERENCIA GENETICA
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
RESISTANCE AUX MALADIES
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTENCIA A LA ENFERMEDAD
RESISTENCIA A PRODUCTOS QUIMICOS
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Online AccessGet more information

Cover

More Information
Summary:Glyphosate (N-phosphonomethyl-glycine) is the active ingredient in the nonselective herbicide Roundup. The sensitivity of crop plants to glyphosate has limited its in-season use as a postemergence herbicide. The extension of the use of Roundup herbicide to allow in-season application in major crops such as soybeans [Glycine max (L.) Merr.] would provide new weed control options for farmers. A glyphosate-tolerant soybean line, 40-3-2, was obtained through expression of the bacterial 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EPSPS) enzyme from Agrobacterium sp. strain CP4. Line 40-3-2 is highly tolerant to glyphosate, showing no visual injury after application of up to 1.68 kg acid equivalent (a.e.) ha-1 of glyphosate under field conditions. Molecular characterization studies determined that the single genetic insert in line 40-3-2 contains only a portion of the cauliflower mosaic virus 35S promoter (P-E35S), the Petunia hybrida EPSPS chloroplast transit peptide (CTP), the CP4 EPSPS gene, and a portion of the 3' nontranslated region of the nopaline synthase gene (NOS 3') terminator. Inheritance studies have shown that the transgene behaves as a single dominant gene and is stable over several generations
Bibliography:F60
F30
H50
9564879
ISSN:0011-183X
1435-0653
DOI:10.2135/cropsci1995.0011183x003500050032x