Protein dielectrophoresis: Advances, challenges, and applications
Protein dielectrophoresis (DEP) has the potential to play an important role as a manipulation, fractionation, preconcentration, and separation method in bioanalysis and as manipulation tool for nanotechnological applications. The first demonstrations of protein DEP have been reported almost 20 years...
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Published in | Electrophoresis Vol. 34; no. 7; pp. 1085 - 1096 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Germany
Blackwell Publishing Ltd
01.04.2013
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Subjects | |
Online Access | Get full text |
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Summary: | Protein dielectrophoresis (DEP) has the potential to play an important role as a manipulation, fractionation, preconcentration, and separation method in bioanalysis and as manipulation tool for nanotechnological applications. The first demonstrations of protein DEP have been reported almost 20 years ago. Since then various experimental realizations to manipulate proteins by DEP as well as more targeted applications employing protein DEP have been demonstrated. This review summarizes the experimental studies in the field of protein DEP trapping and focusing as well as specific applications in separation, molecular patterning, on bioprobes and biosensors. While a comprehensive theoretical model describing protein DEP is still lacking we also attempt to provide an overview of the factors influencing protein DEP and relate to currently available theoretical models. We further point out the variations in experimental conditions used in the past to study the somewhat 20 proteins as well as the implications of protein molecular structure to the DEP response. |
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Bibliography: | National Institutes of Health ark:/67375/WNG-K0CMNCVS-V National Institute of General Medical Sciences - No. 8R21GM103522-03 istex:B097C2C25243EFD5F0097A44A50B0619A150C42F ArticleID:ELPS4608 National Center for Research Resources - No. 5R21RR025826-03 See the article online to view Figs. 1–3 in colour. Colour Online ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-2 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/elps.201200482 |