Cloning and characterization of the fur gene from Moraxella bovis

A homologue of the ferric uptake regulator gene (fur) was isolated from Moraxella bovis by degenerate polymerase chain reaction and cloning. Fur protein of M. bovis exhibited 72.1% amino acid identity with Acinetobacter calcoaceticus Fur. Western blot analysis showed a decrease of Fur expression in...

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Bibliographic Details
Published inMicrobiology and immunology Vol. 47; no. 6; p. 411
Main Authors Kakuda, Tsutomu, Oishi, Daiki, Tsubaki, Shiro, Takai, Shinji
Format Journal Article
LanguageEnglish
Published Australia 01.01.2003
Subjects
Amino Acid Sequence
Bacterial Proteins - genetics
Base Sequence
Blotting, Western
Cloning, Molecular
DNA, Bacterial - genetics
DNA, Complementary - genetics
Gene Expression Regulation, Bacterial
Genes, Bacterial
Iron - pharmacology
Molecular Sequence Data
Moraxella (Moraxella) bovis - drug effects
Moraxella (Moraxella) bovis - genetics
Open Reading Frames - genetics
Polymerase Chain Reaction
Promoter Regions, Genetic - genetics
Repetitive Sequences, Nucleic Acid
Repressor Proteins - genetics
Sequence Homology, Nucleic Acid
Transcription, Genetic
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Summary:A homologue of the ferric uptake regulator gene (fur) was isolated from Moraxella bovis by degenerate polymerase chain reaction and cloning. Fur protein of M. bovis exhibited 72.1% amino acid identity with Acinetobacter calcoaceticus Fur. Western blot analysis showed a decrease of Fur expression in response to sufficient-iron conditions compared with deficient-iron conditions. An electrophoretic mobility-shift assay indicated that Fur protein binds to DNA fragments containing a putative Fur-box derived from the upstream region of the M. bovis fur gene. Fur of M. bovis may regulate the expression of iron transport systems in response to iron limitation in the environment.
ISSN:0385-5600
DOI:10.1111/j.1348-0421.2003.tb03378.x