Oxygen Microscopy by Two-Photon-Excited Phosphorescence
High‐resolution images of oxygen distributions in microheterogeneous samples are obtained by two‐photon laser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two‐photon absorption (2PA) cross‐section. In this probe, energy is harvested by a 2PA ante...
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Published in | Chemphyschem Vol. 9; no. 12; pp. 1673 - 1679 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
25.08.2008
WILEY‐VCH Verlag Wiley |
Subjects | |
Online Access | Get full text |
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Summary: | High‐resolution images of oxygen distributions in microheterogeneous samples are obtained by two‐photon laser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two‐photon absorption (2PA) cross‐section. In this probe, energy is harvested by a 2PA antenna, which passes excitation onto a phosphorescent metalloporphyrin via intramolecular energy transfer. The 2P LSM allows sectioning of oxygen gradients with near diffraction‐limited resolution, and lifetime‐based acquisition eliminates dependence on the local probe concentration. The technique is validated on objects with a priori known oxygen distributions and applied to imaging of pO2 in cells.
Oxygen distributions are imaged by two‐photon laser scanning microscopy (2P LSM) using a newly developed two‐photon‐enhanced phosphorescent nanoprobe (see figure). 2P LSM allows visualization of oxygen gradients in 3D with near diffraction‐limited resolution, and lifetime‐based measurements eliminate dependence on the local probe concentration. |
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Bibliography: | ark:/67375/WNG-N92025MS-R istex:4A820F3E91F445C6012D16D460B3D3082CBA8A42 ArticleID:CPHC200800296 NIH - No. EB007279; No. HL081273; No. P41-RR001348 |
ISSN: | 1439-4235 1439-7641 |
DOI: | 10.1002/cphc.200800296 |